Emanuel Vacalie , Daniel Preda , Petruta Oancea , Anca Ruxandra Leonties , Ludmila Aricov , Adina Raducan
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引用次数: 0
摘要
漆酶是一种底物特异性较低的酶,在某些情况下,需要有介质的存在才能正常工作。因此,本文研究了在从多色金龟子中分离出的漆酶和漆酶-ABTS 系统的存在下,橙 G、橙红 4 R 和甲基红的降解情况。高浓度的漆酶仅能氧化橙 G 和琼脂 4 R,20 分钟内的脱色程度低于 10%。在存在漆酶-ABTS 系统的情况下,反应速度更快,15 分钟内至少有 40% 的染料被降解。研究人员提出了一个动力学模型,该模型可解释所有染料的酶促氧化作用,包括酶的操作失活步骤。这项研究证明,我们可以使用一个单一的动力学模型来计算漆酶-ABTS 系统中所有染料的氧化过程。
Effective degradation of azo dyes by ABTS (2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) mediated laccase. Kinetic studies
Laccases are enzymes with low substrate specificity that in certain cases require the presence of a mediator in order to work properly. Therefore, this paper investigated the degradation of Orange G, Ponceau 4 R and Methyl Red in the presence of laccase isolated from Trametes versicolor and laccase-ABTS system. Laccase, in high concentration, was able to oxidize only Orange G and Ponceau 4 R, with a degree of decolorization less than 10 % in 20 min. In the presence of laccase-ABTS system, the reaction is much faster, and at least 40 % of dyes were degraded in 15 min. A kinetic model accounting for enzymatic oxidation of all dyes, including a step for enzyme operational inactivation, was proposed. This study proves that we can use a single kinetic model for the oxidation of all dyes in the laccase-ABTS system.
期刊介绍:
Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.