{"title":"依赖于腔肠素的荧光素酶：特性及在分子分析中的应用","authors":"L. A. Frank, V. V. Krasitskaya","doi":"10.3103/S0027131424700184","DOIUrl":null,"url":null,"abstract":"<p>This review presents the data on the structure of bioluminescent systems of marine animals that use coelenterazine-dependent luciferases as light-emitting proteins. It is shown that these luciferases and their genetic variants with new useful properties are successfully used as reporter molecules in a variety of analytical systems in vitro and in vivo due to the availability, stability, and high quantum yield of the reactions. Their application ensures a high level of sensitivity, simple design, and fast analysis.</p>","PeriodicalId":709,"journal":{"name":"Moscow University Chemistry Bulletin","volume":"79 3","pages":"203 - 210"},"PeriodicalIF":0.7000,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Coelenterazine-Dependent Luciferases: Properties and Application in Molecular Analysis\",\"authors\":\"L. A. Frank, V. V. Krasitskaya\",\"doi\":\"10.3103/S0027131424700184\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>This review presents the data on the structure of bioluminescent systems of marine animals that use coelenterazine-dependent luciferases as light-emitting proteins. It is shown that these luciferases and their genetic variants with new useful properties are successfully used as reporter molecules in a variety of analytical systems in vitro and in vivo due to the availability, stability, and high quantum yield of the reactions. Their application ensures a high level of sensitivity, simple design, and fast analysis.</p>\",\"PeriodicalId\":709,\"journal\":{\"name\":\"Moscow University Chemistry Bulletin\",\"volume\":\"79 3\",\"pages\":\"203 - 210\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2024-07-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Moscow University Chemistry Bulletin\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.3103/S0027131424700184\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Moscow University Chemistry Bulletin","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.3103/S0027131424700184","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Coelenterazine-Dependent Luciferases: Properties and Application in Molecular Analysis
This review presents the data on the structure of bioluminescent systems of marine animals that use coelenterazine-dependent luciferases as light-emitting proteins. It is shown that these luciferases and their genetic variants with new useful properties are successfully used as reporter molecules in a variety of analytical systems in vitro and in vivo due to the availability, stability, and high quantum yield of the reactions. Their application ensures a high level of sensitivity, simple design, and fast analysis.
期刊介绍：
Moscow University Chemistry Bulletin is a journal that publishes review articles, original research articles, and short communications on various areas of basic and applied research in chemistry, including medical chemistry and pharmacology.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
