为不确定的艾滋病病毒感染者构建新的辅助诊断模型:蛋白质组学研究。

IF 3.4 2区 医学 Q3 IMMUNOLOGY AIDS Pub Date : 2024-09-01 Epub Date: 2024-07-12 DOI:10.1097/QAD.0000000000003977
Yajun Yan, Rui Yuan, Liping Deng, Hui Hu, Yong Yang, Xien Gui, Rongrong Yang
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引用次数: 0

摘要

导言:窗口期是指艾滋病病毒核酸检测(NAT)有反应,但西方印迹(WB)检测没有结果,这一阶段正受到越来越多的关注。提高窗口期 HIV 高危人群的检测效率对于降低意外传播风险至关重要。本研究的目的是为区分不确定的 HIV 感染病例提供一种额外的策略:方法:根据 WB 随访结果,本研究中的人被分为处于 HIV 窗口期的人和未感染 HIV 的人。使用定量液相色谱串联质谱法(LC-MS/MS)对血浆进行分析,以检测差异表达蛋白(DEPs)。利用富集分析研究了这些 DEPs 的生物学意义。蛋白质-蛋白质相互作用(PPI)分析和 LASSO 回归用于识别关键蛋白质。校准曲线、决策曲线和提名图被用来创建模型:结果:共筛选出 57 个 DEPs,其中 33 个上调,24 个下调。最重要的基因本体(GO)富集项是氧化还原酶活性和血红素结合。氧化还原酶占从各种 DEPs 中鉴定出的 10 种主要蛋白质的一半。由过氧化还原酶-2(P32119)、Band 3 阴离子转运蛋白(P02730)和组蛋白 H2A 类型 1(P0C0S8)组成的辅助诊断模型已经开发出来。混淆矩阵参数的结果表明,这种诊断方法在区分不确定的艾滋病毒感染病例方面具有很强的实用性:结论:通过蛋白质组学鉴定和预测的三个 DEPs 对艾滋病窗口期患者的补充鉴定非常有用。
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The construction of a novel supplementary diagnostic model for patients with indeterminate HIV infection.

Introduction: The window period, defined as HIV nucleic acid test (NAT) reactivity but Western blot (WB) test inconclusive, is garnering more attention. Improving the detection efficiency of HIV high-risk populations in the window period is critical to reducing the risk of unanticipated transmission. The purpose of this study was to create an additional strategy for distinguishing indeterminate HIV infection cases.

Methods: Based on WB follow-up results, the individuals in this study were divided into persons in the HIV window period and persons without HIV. Plasma was analyzed using quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) to detect differentially expressed proteins (DEPs). The biological implications of these DEPs were investigated using enrichment analysis. Protein-protein interaction (PPI) analysis and LASSO regression were used to identify key proteins. The calibration curve, decision curve, and nomogram were utilized to create the model.

Results: Fifty-seven DEPs were screened out, with 33 up-regulated and 24 down-regulated in persons with HIV at window period. The most important Gene Ontology (GO) enrichment items are oxidoreductase activity and heme binding. Oxidoreductases account for half of the 10 main proteins identified from various DEPs. An auxiliary diagnostic model comprised of peroxiredoxin-2 (P32119), band 3 anion transport protein (P02730), and histone H2A type 1 (P0C0S8) was developed. The results of the confusion matrix parameters revealed that this diagnostic approach had strong practicability in distinguishing indeterminate HIV infection cases.

Conclusions: The three DEPs identified and predicted by proteomics are useful for the supplemental identification of persons in the HIV window period.

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来源期刊
AIDS
AIDS 医学-病毒学
CiteScore
5.90
自引率
5.30%
发文量
478
审稿时长
3 months
期刊介绍: ​​​​​​​​​​​​​​​​​Publishing the very latest ground breaking research on HIV and AIDS. Read by all the top clinicians and researchers, AIDS has the highest impact of all AIDS-related journals. With 18 issues per year, AIDS guarantees the authoritative presentation of significant advances. The Editors, themselves noted international experts who know the demands of your work, are committed to making AIDS the most distinguished and innovative journal in the field. Submitted articles undergo a preliminary review by the editor. Some articles may be returned to authors without further consideration. Those being considered for publication will undergo further assessment and peer-review by the editors and those invited to do so from a reviewer pool.
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