Thais Lima Ferreira, Amanda Cavalcante Leitão, Lisandra Juvêncio da Silva, Livia Gurgel do Amaral Valente Sá, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Sarah Alves Barbosa, João Batista de Andrade Neto, Amanda Dias Barbosa, Lara Elloyse Almeida Moreira, Maria Erivanda França Rios, Bruno Coêlho Cavalcanti, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior, Cecília Rocha da Silva
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引用次数: 0
摘要
目的：评估芒果苷对氟康唑耐药的念珠菌属的抗真菌活性。材料与方法：使用肉汤微稀释法评估芒果苷的抗真菌活性，并通过棋盘格法评估其与唑类和两性霉素 B 的相互作用。使用 MTT 比色法评估了芒果苷对念珠菌生物膜的活性，并使用流式细胞仪评估了其可能的作用机制。结果显示芒果苷对对氟康唑耐药的白色念珠菌、热带念珠菌和副丝状念珠菌具有活性，并与唑类和两性霉素 B 具有协同作用。芒果苷提高了抗真菌药对念珠菌生物膜的活性，并导致线粒体膜去极化和磷脂酰丝氨酸外化，提示了细胞凋亡。结论：芒果苷与抗真菌药结合具有抗念珠菌的潜力。
Mangiferin potentiates the activity of antifungal agents against fluconazole-resistant Candida spp.
Aim: To evaluate the antifungal activity of mangiferin against Candida spp. resistant to fluconazole.Materials & methods: The antifungal activity of mangiferin was assessed using broth microdilution and its interaction with azoles and amphotericin B was evaluated by checkerboard. The activity of mangiferin against Candida spp. biofilms was assessed using the MTT colorimetric assay and its possible mechanism of action was evaluated using flow cytometry.Results: Mangiferin showed activity against Candida albicans, Candida tropicalis and Candida parapsilosis resistant to fluconazole and showed synergism with azoles and amphotericin B. Mangiferin increased the activity of antifungals against Candida biofilms and caused depolarization of the mitochondrial membrane and externalization of phosphatidylserine, suggesting apoptosis.Conclusion: mangiferin combined with antifungals has potential against Candida spp.
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