Manon Rigot, Alexa M Bersenas, Shane W Bateman, Shauna L Blois, R Darren Wood
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Potential associations of the difference between pre-FFP and post-FFP HA plasma concentrations with the volume of FFP transfused, the cumulative volume of IV fluids administered during the study period, and the HA concentration in the transfused unit were explored.</p><p><strong>Results: </strong>Concentrations of HA were not significantly different between pre- and post-FFP transfusion measurements. The volume of FFP transfused, the cumulative volume of other IV fluids administered during the study time, and the concentration of HA in the FFP units had no significant effect on the change in HA concentration following FFP transfusion in this study.</p><p><strong>Clinical relevance: </strong>This pilot study did not demonstrate an association between FFP administration and changes in plasma HA concentration. The results of this study may serve to help design future research. 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引用次数: 0
摘要
目的:描述重症患者在服用新鲜冷冻血浆(FFP)后循环透明质酸(HA)浓度的变化:描述重症犬输注新鲜冷冻血浆(FFP)后循环透明质酸(HA)浓度的变化,HA是内皮细胞糖萼降解的生物标志物:方法:在输注 FFP 前(T0)、输注最少 7 mL/kg 的 FFP 后 10 分钟(T10)和 90 分钟(T90)采集血浆样本,测量 HA 浓度。在对枸橼酸磷酸盐葡萄糖抗凝血浆的商用透明质酸测定进行内部验证后,还对输注的 FFP 单位中的透明质酸进行了测定。研究人员探讨了输注 FFP 前和输注 FFP 后 HA 血浆浓度的差异与输注 FFP 量、研究期间静脉输液的累积量以及输液单位中 HA 浓度之间的潜在关联:结果:输注 FFP 前和输注 FFP 后的 HA 浓度差异不大。在本研究中,输注的 FFP 量、研究期间其他静脉输液的累积量以及 FFP 单位中的 HA 浓度对输注 FFP 后 HA 浓度的变化没有显著影响:临床相关性:这项试验性研究并未证明输注 FFP 与血浆 HA 浓度变化之间存在关联。这项研究的结果可能有助于设计未来的研究。一种商用检测方法已通过验证,可用于测量柠檬酸磷酸盐葡萄糖抗凝血浆中的 HA。
Evaluation of the effect of fresh-frozen plasma transfusion on circulating hyaluronic acid concentration in critically ill dogs: a pilot study.
Objective: To describe changes in circulating hyaluronic acid (HA) concentration, a biomarker of endothelial glycocalyx degradation, after administration of fresh-frozen plasma (FFP) in critically ill dogs.
Animals: 12 client-owned dogs receiving an FFP transfusion due to underlying disease.
Methods: Plasma samples were collected for HA concentration measurement pre-FFP transfusion (T0) and 10 minutes (T10) and 90 minutes (T90) following completion of FFP transfusion of a minimum volume of 7 mL/kg. Hyaluronic acid was also measured in the transfused FFP units following in-house validation of a commercial HA assay on citrate phosphate dextrose-anticoagulated plasma. Potential associations of the difference between pre-FFP and post-FFP HA plasma concentrations with the volume of FFP transfused, the cumulative volume of IV fluids administered during the study period, and the HA concentration in the transfused unit were explored.
Results: Concentrations of HA were not significantly different between pre- and post-FFP transfusion measurements. The volume of FFP transfused, the cumulative volume of other IV fluids administered during the study time, and the concentration of HA in the FFP units had no significant effect on the change in HA concentration following FFP transfusion in this study.
Clinical relevance: This pilot study did not demonstrate an association between FFP administration and changes in plasma HA concentration. The results of this study may serve to help design future research. A commercial assay was validated to measure HA in citrate phosphate dextrose-anticoagulated plasma.
期刊介绍:
The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.