与鸡不同,火鸡的非结构性 NS1 蛋白在 H7N1 禽流感病毒的复制和组织滋养过程中并不发挥重要作用。

IF 5.5 1区 农林科学 Q1 IMMUNOLOGY Virulence Pub Date : 2024-12-01 Epub Date: 2024-07-16 DOI:10.1080/21505594.2024.2379371
Maryna Kuryshko, Maria Landmann, Christine Luttermann, Reiner Ulrich, Elsayed M Abdelwhab
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引用次数: 0

摘要

高致病性禽流感病毒(AIV)给全球家禽业造成了巨大的经济损失。虽然鸡和火鸡是近缘的胆形目动物,但由于火鸡对 AIV 感染的易感性很高,因此被认为是 AIV 从野鸟适应家禽的桥梁宿主。高致病性禽流感病毒(HPAIV)在家禽中流通后,通过不同病毒蛋白的突变从低致病性(LP)禽流感病毒演变而来,其中包括非结构蛋白(NS1),这是禽流感病毒的主要干扰素(IFN)拮抗剂。目前,高致病性禽流感病毒的致病决定因素在火鸡和鸡中是否相同还不得而知。此前,我们曾发现 HPAIV H7N1 的 NS1 基因突变可显著降低病毒在鸡体内外的复制。在此,我们研究了火鸡接种携带自然截短野生型 NS1(长度为 224 个氨基酸 "aa")或与 LP H7N1 祖先相似的长度为 230 个氨基酸 "aa "的扩展 NS1 的重组 H7N1 后,NS1 对 HPAIV H7N1 病毒复制和毒力的影响。在多周期病毒复制或 NS1 在细胞培养中阻断 IFN 诱导的效率方面没有明显差异。同样,所有病毒在火鸡体内的毒力都很强,在从接种火鸡身上收集的各种器官和拭子中的复制水平相似。这些结果表明,NS1 在高致病性禽流感病毒 H7N1 对火鸡的毒力或复制中不起作用,并进一步表明高致病性禽流感病毒的遗传决定因素在这两种密切相关的胆形动物中有所不同。
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In turkeys, unlike chickens, the non-structural NS1 protein does not play a significant role in the replication and tissue tropism of the H7N1 avian influenza virus.

The economic losses caused by high pathogenicity (HP) avian influenza viruses (AIV) in the poultry industry worldwide are enormous. Although chickens and turkeys are closely related Galliformes, turkeys are thought to be a bridging host for the adaptation of AIV from wild birds to poultry because of their high susceptibility to AIV infections. HPAIV evolve from low pathogenicity (LP) AIV after circulation in poultry through mutations in different viral proteins, including the non-structural protein (NS1), a major interferon (IFN) antagonist of AIV. At present, it is largely unknown whether the virulence determinants of HPAIV are the same in turkeys and chickens. Previously, we showed that mutations in the NS1 of HPAIV H7N1 significantly reduced viral replication in chickens in vitro and in vivo. Here, we investigated the effect of NS1 on the replication and virulence of HPAIV H7N1 in turkeys after inoculation with recombinant H7N1 carrying a naturally truncated wild-type NS1 (with 224 amino-acid "aa" in length) or an extended NS1 with 230-aa similar to the LP H7N1 ancestor. There were no significant differences in multiple-cycle viral replication or in the efficiency of NS1 in blocking IFN induction in the cell culture. Similarly, all viruses were highly virulent in turkeys and replicated at similar levels in various organs and swabs collected from the inoculated turkeys. These results suggest that NS1 does not play a role in the virulence or replication of HPAIV H7N1 in turkeys and further indicate that the genetic determinants of HPAIV differ in these two closely related galliform species.

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来源期刊
Virulence
Virulence IMMUNOLOGY-MICROBIOLOGY
CiteScore
9.20
自引率
1.90%
发文量
123
审稿时长
6-12 weeks
期刊介绍: Virulence is a fully open access peer-reviewed journal. All articles will (if accepted) be available for anyone to read anywhere, at any time immediately on publication. Virulence is the first international peer-reviewed journal of its kind to focus exclusively on microbial pathogenicity, the infection process and host-pathogen interactions. To address the new infectious challenges, emerging infectious agents and antimicrobial resistance, there is a clear need for interdisciplinary research.
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