原位共沉积合成具有纤维内矿化的胶原-黄芪多糖复合材料,作为潜在的仿生骨修复材料。

IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Regenerative Biomaterials Pub Date : 2024-06-21 eCollection Date: 2024-01-01 DOI:10.1093/rb/rbae070
Han Li, Ziying Guan, Liren Wei, Jian Lu, Yanfei Tan, Qingrong Wei
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引用次数: 0

摘要

具有仿骨组织成分和结构的混合材料是骨缺损修复的理想复合材料。受原生骨微观结构的启发,本研究利用原位共沉淀法体外合成了一种功能性矿化胶原纤维(MCF)材料,该方法设计在黄芪多糖(APS)存在的情况下进行,从而在生物矿化胶原-黄芪多糖(MCAPS)纤维内实现了APS负载。透射电子显微镜(TEM)、选区电子衍射(SAED)和扫描电子显微镜(SEM)确定了 MCAPS 纤维的纤维内矿化细节,几乎模仿了骨组织微观结构的二级水平。在所有胶原纤维上和纤维内形成了相对均匀和连续的矿物层,矿物相被鉴定为典型的弱结晶羟基磷灰石(HA),Ca/P 比值约为 1.53。MCAPS 能显著促进骨髓间充质干细胞(BMSC)和小鼠胚胎成骨细胞前体细胞(MC3T3-E1)的增殖。至于 MCAPS 的成骨特性,在 BMSC 和 MC3T3-E1 中检测到碱性磷酸酶(ALP)活性和钙结节(CN)数量明显增加。通过反转录定量聚合酶链反应(RT-qPCR)证实了 RUNX-2、BMP-2 和 OCN 三个成骨相关基因的上调,进一步验证了 MCAPS 对成骨性能的促进作用。14 天的培养结果表明,MCAPS-L 在提高 ALP 活性和 CN 数量以及促进成骨相关基因表达方面的功效优于 MCAPS-M 和 MCAPS-H,这表明 MCAPS 材料中较低剂量的 APS 更适合其促进成骨的特性。
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In situ co-deposition synthesis for collagen-Astragalus polysaccharide composite with intrafibrillar mineralization as potential biomimetic-bone repair materials.

A hybrid material possessing both componential and structural imitation of bone tissue is the preferable composites for bone defect repair. Inspired by the microarchitecture of native bone, this work synthesized in vitro a functional mineralized collagen fibril (MCF) material by utilizing the method of in situ co-precipitation, which was designed to proceed in the presence of Astragalus polysaccharide (APS), thus achieving APS load within the biomineralized collagen-Astragalus polysaccharide (MCAPS) fibrils. Transmission electron microscope (TEM), selected area electron diffraction (SAED) and scanning electronic microscopy (SEM) identified the details of the intrafibrillar mineralization of the MCAPS fibrils, almost mimicking the secondary level of bone tissue microstructure. A relatively uniform and continuous mineral layer formed on and within all collagen fibrils and the mineral phase was identified as typical weak-crystalline hydroxyapatite (HA) with a Ca/P ratio of about 1.53. The proliferation of bone marrow-derived mesenchymal stem cells (BMSC) and mouse embryo osteoblast precursor cells (MC3T3-E1) obtained a significant promotion by MCAPS. As for the osteogenic properties of MCAPS, a distinct increase in the alkaline phosphatase (ALP) activity and the number of calcium nodules (CN) in BMSC and MC3T3-E1 was detected. The up-regulation of three osteogenic-related genes of RUNX-2, BMP-2 and OCN were confirmed via reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to further verify the osteogenic performance promotion of MCAPS. A period of 14 days of culture demonstrated that MCAPS-L exhibited a preferable efficacy in enhancing ALP activity and CN quantity, as well as in promoting the expression of osteogenic-related genes over MCAPS-M and MCAPS-H, indicating that a lower dose of APS within the material of MCAPS is more appropriate for its osteogenesis promotion properties.

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来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
期刊最新文献
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