{"title":"环型 E3 连接酶 PROTEOLYSIS1(来自 Syntrichia caninervis)靶向降解 ABI3 并调节植物胁迫反应","authors":"","doi":"10.1016/j.envexpbot.2024.105893","DOIUrl":null,"url":null,"abstract":"<div><p>Ubiquitin-mediated protein degradation is crucial for plants to respond to environmental stresses, with E3 ligases being particularly important in regulating responses to abiotic stresses. This study focused on the role of E3 ubiquitin ligase in osmotic stress response in desiccation-tolerant mosses. We identified PROTEOLYSIS1 (PRT1), a RING-type E3 ligase, in <em>Syntrichia caninervis</em>. Our <em>in vivo</em> and <em>in vitro</em> assays confirmed that ScPRT1 actively targets the abscisic acid-insensitive 3 (ABI3) protein for degradation. Overexpressing ScPRT1 in <em>Arabidopsis</em> and <em>S. caninervis</em> reduced their tolerance to salt and drought stresses. Transcriptome analysis showed that ScPRT1 overexpression downregulates ABI3 and suppresses genes in the <em>ERFs</em> and <em>DREBs</em> subfamilies, leading to impaired stress resistance. This research enhances our understanding of abiotic stress responses in bryophytes and highlights the significance of protein ubiquitination in these plants.</p></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":null,"pages":null},"PeriodicalIF":4.5000,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"RING-type E3 ligase PROTEOLYSIS1 from Syntrichia caninervis targets ABI3 for degradation and modulates plant stress responses\",\"authors\":\"\",\"doi\":\"10.1016/j.envexpbot.2024.105893\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Ubiquitin-mediated protein degradation is crucial for plants to respond to environmental stresses, with E3 ligases being particularly important in regulating responses to abiotic stresses. This study focused on the role of E3 ubiquitin ligase in osmotic stress response in desiccation-tolerant mosses. We identified PROTEOLYSIS1 (PRT1), a RING-type E3 ligase, in <em>Syntrichia caninervis</em>. Our <em>in vivo</em> and <em>in vitro</em> assays confirmed that ScPRT1 actively targets the abscisic acid-insensitive 3 (ABI3) protein for degradation. Overexpressing ScPRT1 in <em>Arabidopsis</em> and <em>S. caninervis</em> reduced their tolerance to salt and drought stresses. Transcriptome analysis showed that ScPRT1 overexpression downregulates ABI3 and suppresses genes in the <em>ERFs</em> and <em>DREBs</em> subfamilies, leading to impaired stress resistance. This research enhances our understanding of abiotic stress responses in bryophytes and highlights the significance of protein ubiquitination in these plants.</p></div>\",\"PeriodicalId\":11758,\"journal\":{\"name\":\"Environmental and Experimental Botany\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-07-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental and Experimental Botany\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S009884722400251X\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental and Experimental Botany","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S009884722400251X","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
泛素介导的蛋白质降解对植物应对环境胁迫至关重要,其中E3连接酶在调节非生物胁迫响应方面尤为重要。本研究的重点是 E3 泛素连接酶在耐干燥苔藓的渗透胁迫响应中的作用。我们在 Syntrichia caninervis 中发现了一种 RING 型 E3 连接酶 PROTEOLYSIS1(PRT1)。我们的体内和体外实验证实,ScPRT1 能主动靶向降解脱落酸不敏感 3(ABI3)蛋白。在拟南芥和 S. caninervis 中过表达 ScPRT1 会降低它们对盐和干旱胁迫的耐受性。转录组分析表明,ScPRT1过表达会下调ABI3,抑制ERFs和DREBs亚家族的基因,导致抗逆性受损。这项研究加深了我们对叶绿体植物非生物胁迫响应的了解,并强调了蛋白质泛素化在这些植物中的重要意义。
RING-type E3 ligase PROTEOLYSIS1 from Syntrichia caninervis targets ABI3 for degradation and modulates plant stress responses
Ubiquitin-mediated protein degradation is crucial for plants to respond to environmental stresses, with E3 ligases being particularly important in regulating responses to abiotic stresses. This study focused on the role of E3 ubiquitin ligase in osmotic stress response in desiccation-tolerant mosses. We identified PROTEOLYSIS1 (PRT1), a RING-type E3 ligase, in Syntrichia caninervis. Our in vivo and in vitro assays confirmed that ScPRT1 actively targets the abscisic acid-insensitive 3 (ABI3) protein for degradation. Overexpressing ScPRT1 in Arabidopsis and S. caninervis reduced their tolerance to salt and drought stresses. Transcriptome analysis showed that ScPRT1 overexpression downregulates ABI3 and suppresses genes in the ERFs and DREBs subfamilies, leading to impaired stress resistance. This research enhances our understanding of abiotic stress responses in bryophytes and highlights the significance of protein ubiquitination in these plants.
期刊介绍:
Environmental and Experimental Botany (EEB) publishes research papers on the physical, chemical, biological, molecular mechanisms and processes involved in the responses of plants to their environment.
In addition to research papers, the journal includes review articles. Submission is in agreement with the Editors-in-Chief.
The Journal also publishes special issues which are built by invited guest editors and are related to the main themes of EEB.
The areas covered by the Journal include:
(1) Responses of plants to heavy metals and pollutants
(2) Plant/water interactions (salinity, drought, flooding)
(3) Responses of plants to radiations ranging from UV-B to infrared
(4) Plant/atmosphere relations (ozone, CO2 , temperature)
(5) Global change impacts on plant ecophysiology
(6) Biotic interactions involving environmental factors.