Lukas Prielinger, S. Bandyopadhyay, Eva Ortner, Martin Novak, Tanja Radusin, Steffen Annfinsen, N. Sharmin, Bernhard Rainer, M. K. Pettersen
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引用次数: 0
摘要
为了实现欧洲绿色交易目标和实施循环经济,迫切需要提高包装材料的回收率。然而,在将回收材料用于食品接触应用之前,它们必须达到很高的安全标准。根据欧洲食品安全局(EFSA)的规定,必须采用最坏情况假设,并对未知物质的潜在遗传毒性进行评估。艾姆斯试验可检测直接的 DNA 反应效应,它与色谱分析相结合,很有希望对风险评估起到补充作用。本研究旨在评估功能性屏障在十种不同样品(包括原生和回收的低密度聚乙烯箔)中的有效性。傅立叶变换红外分析显示,原生薄膜和回收薄膜之间并无重大差异。光学显微镜显示出质量上的差异和颗粒数量的增加。气相色谱-质谱分析检测并量化了 35 种物质,包括 8 种未知物质。使用微型化的艾姆斯测试法,10 个样品中有 4 个在稀释到 12.5% 的两个迁移测试中呈阳性。所有原生低密度聚乙烯材料的测试结果均为阴性;但回收材料 F 的诱变活性有所提高,诱变倍数高达 28 倍。带有功能性屏障的样品降低了迁移性和诱变性。不过,还需要进一步调查,以确定可能的污染源。
Investigation of the Effectiveness of Barrier Layers to Inhibit Mutagenic Effects of Recycled LDPE Films, Using a Miniaturized Ames Test and GC-MS Analysis
To fulfil the European Green Deal targets and implement a circular economy, there is an urgent need to increase recycling rates of packaging materials. However, before recycled materials can be used in food contact applications, they must meet high safety standards. According to the European Food Safety Authority (EFSA), a worst-case scenario must be applied and unknown substances must be evaluated as being potentially genotoxic. The Ames test, which detects direct DNA-reactive effects, together with chromatographic analysis is very promising to complement risk assessment. This study aims to evaluate the effectiveness of functional barriers in ten different samples, including virgin and recycled LDPE foils. FT-IR analysis did not show major differences between virgin and recycled films. Light microscopy revealed differences in quality and an increased number of particles. GC-MS analysis detected and quantified 35 substances, including eight unknowns. Using a miniaturized version of the Ames test, four of ten samples tested positive in two individual migrates up to a dilution of 12.5%. All virgin LDPE materials tested negative; however, recycled material F showed an increased mutagenic activity, with an n-fold induction up to 28. Samples with functional barriers lowered migration and reduced mutagenicity. Nonetheless, further investigations are needed to identify possible sources of contamination.