黄芩苷绿色生物分子在耐甲氧西林金黄色葡萄球菌小鼠乳腺炎模型中的疗效

IF 0.7 Q4 MICROBIOLOGY Journal of Pure and Applied Microbiology Pub Date : 2024-07-12 DOI:10.22207/jpam.18.3.04
Srishti Soni, Reena Mukherjee, Ujjwal Kumar De, Deeksha Bharti, Mamta Singh, B. R. Paul, Varun Kumar Sarkar, K. Sharun, N. Barkathullah, M. Saminathan
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引用次数: 0

摘要

耐甲氧西林金黄色葡萄球菌(MRSA)乳腺炎对甲氧西林和其他β-内酰胺类抗生素产生耐药性,往往导致治疗失败,因此对全世界的奶牛场构成重大威胁。因此,迫切需要安全有效的替代治疗方法。最近的研究突显了黄芩素的潜力,黄芩素是一种天然类黄酮,以其强大的消炎和抗菌特性而闻名,特别是它与β-内酰胺类抗生素对 MRSA 的协同作用。然而,黄芩苷有限的可溶性和生物利用度阻碍了它在生物医学上的应用。本研究利用 MRSA 引起的小鼠乳腺炎模型,评估了壳聚糖中包裹的黄芩苷与 β-内酰胺类抗生素形成三络合物的治疗效果。实验设计包括七组,每组六只小鼠。我们评估了各种治疗方案缓解 MRSA 感染引起的组织病理学改变和细菌负荷的能力,旨在阐明其潜在机制。我们的研究结果表明,三联疗法能显著减少乳腺组织中的细菌负荷,保持组织的完整性,从而减少接种MRSA后的炎症反应。此外,三联疗法还能明显降低血液中白细胞和中性粒细胞的平均数量,抑制基质金属蛋白酶-9(MMP-9)浓度和 C 反应蛋白(CRP)反应。值得注意的是,黄芩苷与阿莫西林之间的协同作用尤为明显。我们的研究结果表明,壳聚糖包裹的黄芩素与β-内酰胺类抗生素结合有望成为治疗 MRSA 引起的乳腺炎的一种选择。要全面评估其临床可行性,还需要进一步的研究,尤其是在目标动物物种中的研究。
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Therapeutic Efficacy of Baicalein Green Biomolecule in Methicillin-Resistant Staphylococcus aureus Murine Mastitis Model
Methicillin-resistant Staphylococcus aureus (MRSA) mastitis poses a significant threat to dairy herds worldwide, given its resistance to methicillin and other β-lactam antibiotics, which often leads to treatment failure. Consequently, there is an urgent need for safe and effective alternative therapeutic approaches. Recent investigations have highlighted the potential of baicalein, a natural flavonoid known for its potent anti-inflammatory and antibacterial properties, especially its synergistic effects with β-lactam antibiotics against MRSA. However, the limited solubility and bioavailability of baicalein hinder its biomedical utility. The present study assessed the therapeutic efficacy of encapsulated baicalein in chitosan, forming a tricomplex with a β-lactam antibiotic, using a murine model of MRSA-induced mastitis. The experimental design comprised seven groups, each consisting of six mice. We evaluated the ability of various treatment regimens to mitigate histopathological alterations and bacterial burden induced by MRSA infection, aiming to elucidate underlying mechanisms. Our results revealed that tricomplex treatment significantly reduced bacterial load in mammary tissue and preserved tissue integrity, resulting in decreased inflammatory responses post-MRSA inoculation. In addition, tricomplex treatment markedly reduced mean leukocyte and neutrophil counts in blood and suppressed the matrix metalloproteinase-9 (MMP-9) concentration and C-reactive protein (CRP) response. Notably, the synergistic interaction between baicalein and amoxicillin was particularly pronounced. Our findings suggest that chitosan-encapsulated baicalein combined with a β-lactam antibiotic holds promise as a therapeutic option for MRSA-induced mastitis. Further investigations, particularly in target animal species, are warranted to comprehensively evaluate its clinical feasibility.
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来源期刊
Journal of Pure and Applied Microbiology
Journal of Pure and Applied Microbiology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
2.00
自引率
0.00%
发文量
266
审稿时长
11 months
期刊介绍: Journal of Pure and Applied Microbiology (JPAM) is a peer-reviewed, open access international journal of microbiology aims to advance and disseminate research among scientists, academics, clinicians and microbiologists around the world. JPAM publishes high-quality research in all aspects of microbiology in both online and print form on quarterly basis.
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