高粱[Sorghum Bicolor L. Moench]种质中牧草产量和生化性状的遗传差异评估

Santosh, P. K. Pandey
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摘要

本研究于 2018 年 Kharif 季节在潘特纳加的 GBPUAT 进行,在正常播种条件下对 280 个高粱种质品系进行了扩增区组设计。观察记录了不同的产量贡献性状,如开花天数、株高、叶片数、茎杆粗度等;品质性状,如蛋白质含量、总可溶性固形物、体内干物质消化率等;生化性状,如纤维素含量、二氧化硅含量和半纤维素含量等。遗传多样性的统计分析采用分层聚类分析法。分层聚类分析结果表明,高粱种质在不同的产量相关性状、品质性状和生化性状方面存在着显著的遗传多样性。280 个种质品系被分为 XI 个不同的非重叠聚类。第 I 群(52 个)的基因型数量最多,而第 XI 群(1 个)的基因型数量最少。簇-I 的簇内距离最大(52.381),而簇-XI 的簇内距离最小(0.000)。簇内距离大的簇表明,这些簇中的基因型比其他簇内距离小的簇中的基因型具有更高的遗传多样性。在第 IV 和第 XI 聚类间观察到的最大聚类间距(346.854)表明这两个聚类的成员之间的关系较远,这两个聚类的成员杂交后将在分离世代中产生更多的遗传多样性,而在第 VIII 和第 IX 聚类间观察到的最小聚类间距(46.803)表明这两个聚类之间的关系较近,基因型之间的遗传多样性程度较低。本研究筛选出的基因型之间存在大量遗传多样性,这表明该材料可作为杂交计划选择不同亲本的良好来源。为了提高在分离世代中分离出优良变异分离株的可能性,最好尝试在属于群间距离较大的不同基因型之间进行杂交。
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Assessment of Genetic Divergence for Forage Yield and Biochemical Traits in Sorghum [Sorghum Bicolor L. Moench] Germplasm
The present investigation was carried out with two hundred eighty sorghum germplasm lines in augmented block design at GBPUAT, Pantnagar under normal sown condition during the Kharif season 2018. The observations were recorded on different yield contributing traits such as days to flowering, plant height, number of leaves; stem girthetc., quality traits such as protein content, total soluble solids, in vivo dry matter digestibility etc., and biochemical traits like cellulose content, silica content, and hemicelluloses etc. The statistical analysis for genetic diversity was done using hierarchical cluster analysis. The hierarchical cluster analysis revealed that significant amount of genetic diversity was present in sorghum germplasm with respect to different yield relate traits, quality traits and biochemical traits. The 280 germplasm lines were grouped into XI distinct non-overlapping clusters. The cluster-I (52) consisted of highest number of genotypes whereas lowest numbers of genotypes were exhibited by cluster-XI (1). The highest intra-cluster distance was exhibited by cluster-I (52.381) whereas lowest intra-cluster distance was exhibited by cluster-XI (0.000). The clusters with high intra-cluster distances suggested that genotypes in these clusters were more genetic diverse than the genotypes in other clusters with low intra-cluster distances. The maximum inter-cluster distance was observed between clusters-IV and XI (346.854) suggested distant relationship between members of these two clusters and upon crossing the members of these two clusters will give more genetic diversity in segregating generation whereas minimum inter-cluster distance was observed between clusters-VIII and IX (46.803) suggested a closer relationship between these two clusters and low degree of genetic diversity among the genotypes. Presence of substantial genetic diversity among the genotypes screened in the present study indicated that this material may serve as a good source for selecting the diverse parents for hybridization programme. In order to increase the possibility of isolating good trangressive segregants in the segregating generations it would be logical to attempt crosses between the diverse genotypes belonging to clusters separated by large inter-cluster distances.
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