超声波处理辅助提取向日葵蛋白

I. V. Krylova, A. V. Fedorov, M. Domoroshchenkova, T. F. Demyanenko, L. O. Shaginova
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摘要

向日葵粉是一种很有前景的蛋白质来源,可用作肉类、面粉和糖果产品配方中的食品配料。碱性萃取技术是获取向日葵蛋白的传统方法,但使用超声波、微波辐射、高压萃取等物理方法可以提高其效率。本文论述了如何应用超声波处理提高从脱脂植物材料中提取蛋白质的效率:从葵花籽粕和葵花籽粉中提取蛋白质。提取蛋白质的试验样品是在温度为 24-28 摄氏度、频率为 40 赫兹的超声波浴中初步处理 15 分钟后制备的。然后通过碱提取从原料中分离出蛋白质,再进行等电沉淀。与对照样品相比,蛋白质制备分离的粗蛋白含量更高(93.66% m.f.b)。蛋白质的质量产率为原料中蛋白质含量的 64%。超声波处理对从不同粗蛋白含量的原料中提取蛋白质效率的影响得到了证实。研究结果表明了使用超声波处理分离向日葵蛋白质的可行性。特别是,与对照样品相比,经过超声波处理的试验蛋白质样品中的粗蛋白含量提高了 8.23%。与其他研究结果相比,所获得的结果具有一致性。然而,关于使用超声波提取向日葵衍生产品的研究数量有限。然而,关于使用超声波提取向日葵加工产品的研究为数不多。
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Ultrasonic treatment assisted extraction of sunflower protein
Sunflower meal is a promising source of protein, which can be used as a food ingredient in the formulations of meat, flour and confectionery products. Alkaline extraction technology is traditionally used to obtain sunflower protein, but its efficiency can be increased using physical methods: ultrasound, microwave radiation, extraction at high pressure and others. Thу article deals with an application of the ultrasonic treatment for increase of the efficiency of protein extraction from defatted plant material: from the protein fraction of sunflower meal and from sunflower meal. The trial samples for protein extraction were prepared by the preliminary treatment of the samples in the ultrasonic bath for 15 minutes at a frequency of 40 hertz at temperature 24-28° C. The control sample was not subjected to such pretreatment. Then protein was isolated from the raw material by alkaline extraction followed by isoelectric precipitation. The possibility of the protein preparation isolation with a higher crude protein content (93,66% m.f.b) compared to the control sample has been shown. The mass yield of protein was 64% of its content in the raw material. The influence of ultrasonic treatment on the efficiency of protein extraction from raw material with different crude protein content is demonstrated. The results of the study show the feasibility of usage of ultrasound treatment in isolation of sunflower protein. In particular, the crude protein content in the trial protein sample after ultrasonic treatment was increased by 8,23% compared to the control sample. The obtained results demonstrated their consistency when compared to the results of other studies. However, there are only a limited number of studies on the use of ultrasound in the extraction of sunflower derived products.A comparison of the results obtained showed their consistency with the results of other studies. However, there are only a small number of studies on the use of ultrasound in the extraction of sunflower processing products.
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