Quantitative Evaluation of The Power of Amelogenin and Chitosan in Natural Repair of Dentin Defects Using Energy‑Dispersive Analytical X‑ray Element Analysis

Objectives: Evaluate the potential of amelogenin and chitosan to repair eroded dentin at different times by means of a chemical element analysis. Methodology : Twelve human lower first molars were collected. Radicular part was removed, while coronal part was sectioned mesiodistally. Two dentin slabs were obtained from each molar. Samples were immersed in Coca Cola beverage (Coca-Cola® Co., Egypt) for 25 hours. Samples in group I were stored in artificial saliva, while in group II they were treated with agarose hydrogel. Agarose hydrogel was loaded with chitosan in group III and with Emdogain in group IV. Hydrogels were applied to samples for 5 hours. This procedure was repeated daily for 15 days. Dentin mineral contents (Ca & P) were tested using energy dispersive analytical X-ray. Results: The baseline mean values of Ca & P in sound dentin samples were (23.08±2.23) & (7.91±3.09), while were significantly decreased into (6.88±2.30) & (4.04±1.28) after immersion in Coca Cola. On comparing Ca & P contents of different groups after 5 days treatment, ANOVA showed no significant differences in both elements, while after 10 days a highly statistically significant differences were recorded in all groups (p=0.000). After 15 days, a highly significant differences in Ca content were recorded (p=0.000), while no significant differences were recorded in P content (p=0.167). Emdogain group represented the best results. Conclusion: Emdogain exhibits promising remineralizing effect on erosive dentin, as it has the highest mean difference when compared to chitosan and control groups.