Erzhan Zh Kidiraliyev , Ainur A. Zhaksylykova , Eleonora A. Kydyrkhanova , Kaissar Zh Kushaliyev , Alexandr Shevtsov , Ryskeldina Anara , Zhibek P. Sembaeva , M.A. Arzybaev , Christine M. Budke , Aida M. Abdybekova
{"title":"对哈萨克斯坦共和国伏尔加-乌拉尔塞加羚羊种群艾美耳病感染情况的分子评估","authors":"Erzhan Zh Kidiraliyev , Ainur A. Zhaksylykova , Eleonora A. Kydyrkhanova , Kaissar Zh Kushaliyev , Alexandr Shevtsov , Ryskeldina Anara , Zhibek P. Sembaeva , M.A. Arzybaev , Christine M. Budke , Aida M. Abdybekova","doi":"10.1016/j.ijppaw.2024.100966","DOIUrl":null,"url":null,"abstract":"<div><p>Saiga antelope (<em>Saiga tatarica</em>) is a protected species in Kazakhstan. Little is known about the parasitofauna of these mammals. Therefore, the focus of this study was to evaluate the prevalence and species diversity of <em>Eimeria</em> spp. infection in the Volga-Ural Saiga antelope population. In June 2023, 104 Saiga antelope fecal samples collected from the district of Zhanibek, located in the province of West Kazakhstan were evaluated using microscopic and molecular techniques. Based on coprovoscopy results, <em>Eimeria</em> spp. Oocysts were present in 22 samples (21%). The four fecal samples containing the largest numbers of <em>Eimeria</em> spp. Oocysts per 10x field were selected for further genetic analysis. DNA extraction, nested PCR amplification, and sequencing were performed on 91 clones, with 80 clones forming a distinct clade and exhibiting genetic similarity to MT801034 Ei<em>meria</em> sp. Voucher HY3. These clones possibly represent an <em>Eimeria</em> specific to Saiga antelopes and gazelle that has previously been morphologically described as <em>Eimeria elegans</em> (Svanbaev, 1979), underscoring the importance of further research into parasitic infections in this protected species.</p></div>","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"24 ","pages":"Article 100966"},"PeriodicalIF":2.0000,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213224424000622/pdfft?md5=1bfc6c3b623bab3d9b3cca7d9c6ce1cb&pid=1-s2.0-S2213224424000622-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Molecular evaluation of Eimeria spp. Infection in the Volga-Ural Saiga antelope population of the Republic of Kazakhstan\",\"authors\":\"Erzhan Zh Kidiraliyev , Ainur A. Zhaksylykova , Eleonora A. Kydyrkhanova , Kaissar Zh Kushaliyev , Alexandr Shevtsov , Ryskeldina Anara , Zhibek P. Sembaeva , M.A. Arzybaev , Christine M. Budke , Aida M. Abdybekova\",\"doi\":\"10.1016/j.ijppaw.2024.100966\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Saiga antelope (<em>Saiga tatarica</em>) is a protected species in Kazakhstan. Little is known about the parasitofauna of these mammals. Therefore, the focus of this study was to evaluate the prevalence and species diversity of <em>Eimeria</em> spp. infection in the Volga-Ural Saiga antelope population. In June 2023, 104 Saiga antelope fecal samples collected from the district of Zhanibek, located in the province of West Kazakhstan were evaluated using microscopic and molecular techniques. Based on coprovoscopy results, <em>Eimeria</em> spp. Oocysts were present in 22 samples (21%). The four fecal samples containing the largest numbers of <em>Eimeria</em> spp. Oocysts per 10x field were selected for further genetic analysis. DNA extraction, nested PCR amplification, and sequencing were performed on 91 clones, with 80 clones forming a distinct clade and exhibiting genetic similarity to MT801034 Ei<em>meria</em> sp. Voucher HY3. 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Molecular evaluation of Eimeria spp. Infection in the Volga-Ural Saiga antelope population of the Republic of Kazakhstan
Saiga antelope (Saiga tatarica) is a protected species in Kazakhstan. Little is known about the parasitofauna of these mammals. Therefore, the focus of this study was to evaluate the prevalence and species diversity of Eimeria spp. infection in the Volga-Ural Saiga antelope population. In June 2023, 104 Saiga antelope fecal samples collected from the district of Zhanibek, located in the province of West Kazakhstan were evaluated using microscopic and molecular techniques. Based on coprovoscopy results, Eimeria spp. Oocysts were present in 22 samples (21%). The four fecal samples containing the largest numbers of Eimeria spp. Oocysts per 10x field were selected for further genetic analysis. DNA extraction, nested PCR amplification, and sequencing were performed on 91 clones, with 80 clones forming a distinct clade and exhibiting genetic similarity to MT801034 Eimeria sp. Voucher HY3. These clones possibly represent an Eimeria specific to Saiga antelopes and gazelle that has previously been morphologically described as Eimeria elegans (Svanbaev, 1979), underscoring the importance of further research into parasitic infections in this protected species.
期刊介绍：
The International Journal for Parasitology: Parasites and Wildlife (IJP-PAW) publishes the results of original research on parasites of all wildlife, invertebrate and vertebrate. This includes free-ranging, wild populations, as well as captive wildlife, semi-domesticated species (e.g. reindeer) and farmed populations of recently domesticated or wild-captured species (e.g. cultured fishes). Articles on all aspects of wildlife parasitology are welcomed including taxonomy, biodiversity and distribution, ecology and epidemiology, population biology and host-parasite relationships. The impact of parasites on the health and conservation of wildlife is seen as an important area covered by the journal especially the potential role of environmental factors, for example climate. Also important to the journal is ''one health'' and the nature of interactions between wildlife, people and domestic animals, including disease emergence and zoonoses.
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