Chitosan from gladius of Doryteuthis sibogae and their capability to inhibit the blood clotting and its antibacterial effect against human pathogens

Chitosan, a derivative of chitin derived from the gladius of the marine cephalopod Doryteuthis sibogae, has drawn interest in biomedical research because of its possible antibacterial effects on human infections and inhibition of blood clotting. Chitosan was extracted from chitin isolated from the gladius of D. sibogae via demineralization and deproteination. Chitosan was structurally characterized using FT-IR, XRD, and FESEM. Additionally, the disc diffusion method was used to investigate the antibacterial efficacy of chitosan against three human pathogens. Effective antibacterial activity was demonstrated against both Gram-positive and Gram-negative microorganisms. Chitosan is a potent anticoagulant in anticoagulant assays employing activated partial thromboplastin time (APTT) and prothrombin time (PT).