青少年双歧杆菌 BAD_1527 基因编码 AXH-m 型 GH43_22 α-L-阿拉伯呋喃糖苷酶。

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY AMB Express Pub Date : 2024-07-20 DOI:10.1186/s13568-024-01738-9
Walid Fathallah, Vladimír Puchart
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摘要

先前有人认为青春期双歧杆菌基因 BAD_1527 编码一种 β-木糖苷酶(Kobayashi 等人,Mar Drugs 18:174, 2020)。我们使用多种天然和人工底物对 GH43_22 蛋白的底物特异性进行了详细研究,结果表明该酶既不水解线性木寡糖,也不水解葡萄糖醛酸聚糖。只有人工 4-硝基苯基 β-D-木吡喃糖苷(1.58 mU/mg)能释放木糖。相应的 α-L-arabinofuranoside 的底物(2.17 U/mg)要好三个数量级。阿拉伯糖是从阿拉伯木聚糖和α-1,3-或α-1,2-类似阿拉伯糖基的木寡糖中释放出来的唯一单糖。此外,该酶还能有效地去支链甜菜阿拉伯聚糖和单阿拉伯糖基化的α-1,5-L-阿拉伯低聚木糖,尽管短线型α-1,5-L-阿拉伯低聚木糖的降解速度也很慢。另一方面,去支链的阿拉伯聚糖、阿拉伯半乳聚糖以及阿拉伯聚糖和阿拉伯木聚糖的 2,3 双阿拉伯糖基主链残基不能作为底物。因此,BAD_1527 基因编码的酶是一种典型的具有 AXH-m 特异性的 α-L-arabinofuranosidase。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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The Bifidobacterium adolescentis BAD_1527 gene encodes GH43_22 α-L-arabinofuranosidase of AXH-m type.

Bifidobacterium adolescentis gene BAD_1527 has previously been suggested to code for a β-xylosidase (Kobayashi et al., Mar Drugs 18:174, 2020). Our detailed investigation of the substrate specificity of the GH43_22 protein using a wide spectrum of natural and artificial substrates showed that the enzyme hydrolyzed neither linear xylooligosaccharides nor glucuronoxylan. Xylose was released only from the artificial 4-nitrophenyl β-D-xylopyranoside (1.58 mU/mg). The corresponding α-L-arabinofuranoside was by three orders of magnitude better substrate (2.17 U/mg). Arabinose was the only monosaccharide liberated from arabinoxylan and α-1,3- or α-1,2-singly arabinosylated xylooligosaccharides. Moreover, the enzyme efficiently debranched sugar beet arabinan and singly arabinosylated α-1,5-L-arabinooligosaccharides, although short linear α-1,5-L-arabinooligosaccharides were also slowly degraded. On the other hand, debranched arabinan, arabinogalactan as well as 2,3-doubly arabinosylated main chain residues of arabinan and arabinoxylan did not serve as substrates. Thus, the enzyme encoded by the BAD_1527 gene is a typical α-L-arabinofuranosidase of AXH-m specificity.

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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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Optimized production and characterization of a thermostable cellulase from Streptomyces thermodiastaticus strain. Marine chitinase AfChi: green defense management against Colletotrichum gloeosporioides and anthracnose. Antibiotics and lectin C for diarrhea control intervention in piglets and influences. Correction: Improving carboxymethyl cellulose edible coating using ZnO nanoparticles from irradiated Alternaria tenuissima. Detection and molecular characterization of carbapenem-resistant gram-negative bacterial isolates.
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