Minh Tam Le, Trung Van Nguyen, Thai Thanh Thi Nguyen, Hong Nhan Thi Dang, Quoc Huy Vu Nguyen
{"title":"拉伸玻璃毛细管中的无冷冻保护剂精子玻璃化对精子参数和 DNA 完整性的影响：实验室试验研究。","authors":"Minh Tam Le, Trung Van Nguyen, Thai Thanh Thi Nguyen, Hong Nhan Thi Dang, Quoc Huy Vu Nguyen","doi":"10.18502/ijrm.v22i4.16391","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Vitrification is a recently introduced yet widely applied assisted reproduction technique. So far, the effects of the chemicals and devices in vitrification on sperm motility and DNA integrity are still unclear.</p><p><strong>Objective: </strong>This study aimed to examine sperm quality, as determined by semen analysis and sperm DNA integrity when vitrified with or without cryoprotectant agents (CPAs) using pulled-glass capillaries.</p><p><strong>Materials and methods: </strong>Between February and June 2020, 50 infertile men from the Hue Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Vietnam, were enrolled. Sperm samples, prepared using the swim-up technique, were divided into 2 groups: vitrification with CPAs (group 1) and without CPAs (group 2). Vitrified sperm samples were preserved in 10 µL pulled-glass capillaries. Motility, sperm membrane integrity, and the DNA fragmentation index were tested.</p><p><strong>Results: </strong>Sperm motility in vitrified media with CPAs (54.4 <math><mo>±</mo></math> 11%) was statistically higher than in media without CPAs (51.14 <math><mo>±</mo></math> 10.6%, p <math><mo><</mo></math> 0.05). CPAs did not affect sperm membrane integrity or large halo ratio (71.34 <math><mo>±</mo></math> 8.47 vs. 70.38 <math><mo>±</mo></math> 8.11 and 50.84 <math><mo>±</mo></math> 18.92 vs. 51.98 <math><mo>±</mo></math> 19.44, respectively). Group 2 exhibited a lower DNA fragmentation index than group 1 after vitrification (14.2 <math><mo>±</mo></math> 8.47 vs. 12.60 <math><mo>±</mo></math> 9.03, p = 0.021).</p><p><strong>Conclusion: </strong>Using a pulled-glass capillary for sperm vitrification, the presence of CPAs in the vitrification medium resulted in higher progressive motility and lower DNA fragmentation index than the medium without CPAs.</p>","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"22 4","pages":"305-316"},"PeriodicalIF":1.6000,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11255465/pdf/","citationCount":"0","resultStr":"{\"title\":\"Impact of cryoprotectant-free sperm vitrification in pulled-glass capillary on sperm parameters and DNA integrity: A lab trial study.\",\"authors\":\"Minh Tam Le, Trung Van Nguyen, Thai Thanh Thi Nguyen, Hong Nhan Thi Dang, Quoc Huy Vu Nguyen\",\"doi\":\"10.18502/ijrm.v22i4.16391\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Vitrification is a recently introduced yet widely applied assisted reproduction technique. So far, the effects of the chemicals and devices in vitrification on sperm motility and DNA integrity are still unclear.</p><p><strong>Objective: </strong>This study aimed to examine sperm quality, as determined by semen analysis and sperm DNA integrity when vitrified with or without cryoprotectant agents (CPAs) using pulled-glass capillaries.</p><p><strong>Materials and methods: </strong>Between February and June 2020, 50 infertile men from the Hue Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Vietnam, were enrolled. Sperm samples, prepared using the swim-up technique, were divided into 2 groups: vitrification with CPAs (group 1) and without CPAs (group 2). Vitrified sperm samples were preserved in 10 µL pulled-glass capillaries. Motility, sperm membrane integrity, and the DNA fragmentation index were tested.</p><p><strong>Results: </strong>Sperm motility in vitrified media with CPAs (54.4 <math><mo>±</mo></math> 11%) was statistically higher than in media without CPAs (51.14 <math><mo>±</mo></math> 10.6%, p <math><mo><</mo></math> 0.05). CPAs did not affect sperm membrane integrity or large halo ratio (71.34 <math><mo>±</mo></math> 8.47 vs. 70.38 <math><mo>±</mo></math> 8.11 and 50.84 <math><mo>±</mo></math> 18.92 vs. 51.98 <math><mo>±</mo></math> 19.44, respectively). Group 2 exhibited a lower DNA fragmentation index than group 1 after vitrification (14.2 <math><mo>±</mo></math> 8.47 vs. 12.60 <math><mo>±</mo></math> 9.03, p = 0.021).</p><p><strong>Conclusion: </strong>Using a pulled-glass capillary for sperm vitrification, the presence of CPAs in the vitrification medium resulted in higher progressive motility and lower DNA fragmentation index than the medium without CPAs.</p>\",\"PeriodicalId\":14386,\"journal\":{\"name\":\"International Journal of Reproductive Biomedicine\",\"volume\":\"22 4\",\"pages\":\"305-316\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-06-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11255465/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Reproductive Biomedicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijrm.v22i4.16391\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/4/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"OBSTETRICS & GYNECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Reproductive Biomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijrm.v22i4.16391","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/4/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
Impact of cryoprotectant-free sperm vitrification in pulled-glass capillary on sperm parameters and DNA integrity: A lab trial study.
Background: Vitrification is a recently introduced yet widely applied assisted reproduction technique. So far, the effects of the chemicals and devices in vitrification on sperm motility and DNA integrity are still unclear.
Objective: This study aimed to examine sperm quality, as determined by semen analysis and sperm DNA integrity when vitrified with or without cryoprotectant agents (CPAs) using pulled-glass capillaries.
Materials and methods: Between February and June 2020, 50 infertile men from the Hue Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Vietnam, were enrolled. Sperm samples, prepared using the swim-up technique, were divided into 2 groups: vitrification with CPAs (group 1) and without CPAs (group 2). Vitrified sperm samples were preserved in 10 µL pulled-glass capillaries. Motility, sperm membrane integrity, and the DNA fragmentation index were tested.
Results: Sperm motility in vitrified media with CPAs (54.4 11%) was statistically higher than in media without CPAs (51.14 10.6%, p 0.05). CPAs did not affect sperm membrane integrity or large halo ratio (71.34 8.47 vs. 70.38 8.11 and 50.84 18.92 vs. 51.98 19.44, respectively). Group 2 exhibited a lower DNA fragmentation index than group 1 after vitrification (14.2 8.47 vs. 12.60 9.03, p = 0.021).
Conclusion: Using a pulled-glass capillary for sperm vitrification, the presence of CPAs in the vitrification medium resulted in higher progressive motility and lower DNA fragmentation index than the medium without CPAs.
期刊介绍：
The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.
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