Chiara Villa, Valeria Secchi, Mirco Macchi, Luana Tripodi, Elena Trombetta, Desiree Zambroni, Francesco Padelli, Michele Mauri, Monica Molinaro, Rebecca Oddone, Andrea Farini, Antonella De Palma, Laura Varela Pinzon, Federica Santarelli, Roberto Simonutti, PierLuigi Mauri, Laura Porretti, Marcello Campione, Domenico Aquino, Angelo Monguzzi, Yvan Torrente
{"title":"磁场驱动的外泌体靶向调节肌营养不良症肌肉的免疫和代谢变化","authors":"Chiara Villa, Valeria Secchi, Mirco Macchi, Luana Tripodi, Elena Trombetta, Desiree Zambroni, Francesco Padelli, Michele Mauri, Monica Molinaro, Rebecca Oddone, Andrea Farini, Antonella De Palma, Laura Varela Pinzon, Federica Santarelli, Roberto Simonutti, PierLuigi Mauri, Laura Porretti, Marcello Campione, Domenico Aquino, Angelo Monguzzi, Yvan Torrente","doi":"10.1038/s41565-024-01725-y","DOIUrl":null,"url":null,"abstract":"Exosomes are promising therapeutics for tissue repair and regeneration to induce and guide appropriate immune responses in dystrophic pathologies. However, manipulating exosomes to control their biodistribution and targeting them in vivo to achieve adequate therapeutic benefits still poses a major challenge. Here we overcome this limitation by developing an externally controlled delivery system for primed annexin A1 myo-exosomes (Exomyo). Effective nanocarriers are realized by immobilizing the Exomyo onto ferromagnetic nanotubes to achieve controlled delivery and localization of Exomyo to skeletal muscles by systemic injection using an external magnetic field. Quantitative muscle-level analyses revealed that macrophages dominate the uptake of Exomyo from these ferromagnetic nanotubes in vivo to synergistically promote beneficial muscle responses in a murine animal model of Duchenne muscular dystrophy. Our findings provide insights into the development of exosome-based therapies for muscle diseases and, in general, highlight the formulation of effective functional nanocarriers aimed at optimizing exosome biodistribution. Exosome targeting for therapeutic needs remains a challenge. Here, the authors show that ferromagnetic-nanotube-passivated exosomes promote the transition of proinflammatory macrophages to an anti-inflammatory state and myogenic maturation of dystrophic muscle progenitors in a murine model.","PeriodicalId":18915,"journal":{"name":"Nature nanotechnology","volume":null,"pages":null},"PeriodicalIF":38.1000,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41565-024-01725-y.pdf","citationCount":"0","resultStr":"{\"title\":\"Magnetic-field-driven targeting of exosomes modulates immune and metabolic changes in dystrophic muscle\",\"authors\":\"Chiara Villa, Valeria Secchi, Mirco Macchi, Luana Tripodi, Elena Trombetta, Desiree Zambroni, Francesco Padelli, Michele Mauri, Monica Molinaro, Rebecca Oddone, Andrea Farini, Antonella De Palma, Laura Varela Pinzon, Federica Santarelli, Roberto Simonutti, PierLuigi Mauri, Laura Porretti, Marcello Campione, Domenico Aquino, Angelo Monguzzi, Yvan Torrente\",\"doi\":\"10.1038/s41565-024-01725-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Exosomes are promising therapeutics for tissue repair and regeneration to induce and guide appropriate immune responses in dystrophic pathologies. 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Magnetic-field-driven targeting of exosomes modulates immune and metabolic changes in dystrophic muscle
Exosomes are promising therapeutics for tissue repair and regeneration to induce and guide appropriate immune responses in dystrophic pathologies. However, manipulating exosomes to control their biodistribution and targeting them in vivo to achieve adequate therapeutic benefits still poses a major challenge. Here we overcome this limitation by developing an externally controlled delivery system for primed annexin A1 myo-exosomes (Exomyo). Effective nanocarriers are realized by immobilizing the Exomyo onto ferromagnetic nanotubes to achieve controlled delivery and localization of Exomyo to skeletal muscles by systemic injection using an external magnetic field. Quantitative muscle-level analyses revealed that macrophages dominate the uptake of Exomyo from these ferromagnetic nanotubes in vivo to synergistically promote beneficial muscle responses in a murine animal model of Duchenne muscular dystrophy. Our findings provide insights into the development of exosome-based therapies for muscle diseases and, in general, highlight the formulation of effective functional nanocarriers aimed at optimizing exosome biodistribution. Exosome targeting for therapeutic needs remains a challenge. Here, the authors show that ferromagnetic-nanotube-passivated exosomes promote the transition of proinflammatory macrophages to an anti-inflammatory state and myogenic maturation of dystrophic muscle progenitors in a murine model.
期刊介绍:
Nature Nanotechnology is a prestigious journal that publishes high-quality papers in various areas of nanoscience and nanotechnology. The journal focuses on the design, characterization, and production of structures, devices, and systems that manipulate and control materials at atomic, molecular, and macromolecular scales. It encompasses both bottom-up and top-down approaches, as well as their combinations.
Furthermore, Nature Nanotechnology fosters the exchange of ideas among researchers from diverse disciplines such as chemistry, physics, material science, biomedical research, engineering, and more. It promotes collaboration at the forefront of this multidisciplinary field. The journal covers a wide range of topics, from fundamental research in physics, chemistry, and biology, including computational work and simulations, to the development of innovative devices and technologies for various industrial sectors such as information technology, medicine, manufacturing, high-performance materials, energy, and environmental technologies. It includes coverage of organic, inorganic, and hybrid materials.