垂直限制增强了细菌抽动运动的表面探索能力。

IF 4.3 2区 生物学 Q2 MICROBIOLOGY Environmental microbiology Pub Date : 2024-07-22 DOI:10.1111/1462-2920.16679
Xiao Chen, Rongjing Zhang, Junhua Yuan
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引用次数: 0

摘要

细菌通常生活在空间有限的环境中,它们会附着在物体表面。在这些表面上常见的一种运动形式是细菌的抽动运动,其动力来自第四型纤毛的伸缩。虽然对无限制条件下的抽动运动进行了广泛研究,但对空间限制对这种行为的影响还不甚了解。在本研究中,我们探索了单个抽动铜绿假单胞菌细胞在空间受限条件下的扩散特性。我们将细菌置于琼脂糖层和玻璃层之间,然后跟踪单个细胞的长期抽动运动。有趣的是,我们发现,虽然封闭降低了抽动的即时速度,但却增加了扩散。通过结合机械和几何分析以及数值模拟,我们发现扩散的增加可归因于机械因素。琼脂糖施加的限制改变了细菌的扩散模式,从正常扩散变成了超扩散。这些发现为了解细菌在封闭环境中的运动行为提供了宝贵的见解。
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Vertical confinement enhances surface exploration in bacterial twitching motility

Bacteria are often found in environments where space is limited, and they attach themselves to surfaces. One common form of movement on these surfaces is bacterial twitching motility, which is powered by the extension and retraction of type IV pili. Although twitching motility in unrestricted conditions has been extensively studied, the effects of spatial confinement on this behaviour are not well understood. In this study, we explored the diffusive properties of individual twitching Pseudomonas aeruginosa cells in spatially confined conditions. We achieved this by placing the bacteria between layers of agarose and glass, and then tracking the long-term twitching motility of individual cells. Interestingly, we found that while confinement reduced the immediate speed of twitching, it paradoxically increased diffusion. Through a combination of mechanical and geometrical analysis, as well as numerical simulations, we showed that this increase in diffusion could be attributed to mechanical factors. The constraint imposed by the agarose altered the diffusion pattern of the bacteria from normal to superdiffusion. These findings provide valuable insights into the motile behaviour of bacteria in confined environments.

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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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