{"title":"利用新型原位法研究聚苯乙烯微塑料对 Skeletonema costatum 释放的细胞外和细胞内溶解有机物的影响。","authors":"","doi":"10.1016/j.envpol.2024.124604","DOIUrl":null,"url":null,"abstract":"<div><p>Microplastics (MPs) affect the physicochemical algal-dissolved organic matter properties, indirectly influencing the environmental behavior of contaminants including persistent organic pollutants and heavy metals. Limited research is available on the roles played by intracellular- and extracellular-dissolved organic matter (I-DOM and E-DOM) in the processes that affect the environmental behavior of contaminants. Furthermore, the effects of MPs on the production of I-DOM and E-DOM, as well as their environmental behaviors, remain uncertain. A critical issue lies in the challenge of quantitatively identifying I-DOM and E-DOM <em>in situ</em>. In this work, a new fluorescence ratio method was developed and applied to <em>in situ</em> examine the impacts of polystyrene (PS) MPs (50, 500 nm, and 5 μm) on the I-DOM and E-DOM released by <em>Skeletonema costatum</em> (<em>S. costatum</em>). The experimental results indicated that the detection limits were 0.06 mg L<sup>−1</sup>, with the respective minimum detectable proportions being 2% for both E-DOM and I-DOM. The suppressive effects of 10–50 mg L<sup>−1</sup> of 50 and 500 nm PS MPs on the cell proliferation of <em>S. costatum</em> and the E-DOM secretion were most pronounced on day 6. And the rates of suppression of E-DOM secretion were 10.1%–18.2% and 4.2%–13.9%, respectively. The exposure of algal cells to 50 mg L<sup>−1</sup> of 50 and 500 nm PS MPs led to cell rupture and the leakage of I-DOM on day 6. This suggests that the developed method in the laboratory could offer a promising approach for studying the generation of E-DOM and I-DOM <em>in situ</em>, as well as their environmental behaviors affected by MPs.</p></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":null,"pages":null},"PeriodicalIF":7.6000,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of polystyrene microplastics on the extracellular and intracellular dissolved organic matter released by Skeletonema costatum using a novel in situ method\",\"authors\":\"\",\"doi\":\"10.1016/j.envpol.2024.124604\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Microplastics (MPs) affect the physicochemical algal-dissolved organic matter properties, indirectly influencing the environmental behavior of contaminants including persistent organic pollutants and heavy metals. Limited research is available on the roles played by intracellular- and extracellular-dissolved organic matter (I-DOM and E-DOM) in the processes that affect the environmental behavior of contaminants. Furthermore, the effects of MPs on the production of I-DOM and E-DOM, as well as their environmental behaviors, remain uncertain. A critical issue lies in the challenge of quantitatively identifying I-DOM and E-DOM <em>in situ</em>. In this work, a new fluorescence ratio method was developed and applied to <em>in situ</em> examine the impacts of polystyrene (PS) MPs (50, 500 nm, and 5 μm) on the I-DOM and E-DOM released by <em>Skeletonema costatum</em> (<em>S. costatum</em>). The experimental results indicated that the detection limits were 0.06 mg L<sup>−1</sup>, with the respective minimum detectable proportions being 2% for both E-DOM and I-DOM. The suppressive effects of 10–50 mg L<sup>−1</sup> of 50 and 500 nm PS MPs on the cell proliferation of <em>S. costatum</em> and the E-DOM secretion were most pronounced on day 6. And the rates of suppression of E-DOM secretion were 10.1%–18.2% and 4.2%–13.9%, respectively. The exposure of algal cells to 50 mg L<sup>−1</sup> of 50 and 500 nm PS MPs led to cell rupture and the leakage of I-DOM on day 6. This suggests that the developed method in the laboratory could offer a promising approach for studying the generation of E-DOM and I-DOM <em>in situ</em>, as well as their environmental behaviors affected by MPs.</p></div>\",\"PeriodicalId\":311,\"journal\":{\"name\":\"Environmental Pollution\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":7.6000,\"publicationDate\":\"2024-07-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Pollution\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0269749124013186\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Pollution","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0269749124013186","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Effects of polystyrene microplastics on the extracellular and intracellular dissolved organic matter released by Skeletonema costatum using a novel in situ method
Microplastics (MPs) affect the physicochemical algal-dissolved organic matter properties, indirectly influencing the environmental behavior of contaminants including persistent organic pollutants and heavy metals. Limited research is available on the roles played by intracellular- and extracellular-dissolved organic matter (I-DOM and E-DOM) in the processes that affect the environmental behavior of contaminants. Furthermore, the effects of MPs on the production of I-DOM and E-DOM, as well as their environmental behaviors, remain uncertain. A critical issue lies in the challenge of quantitatively identifying I-DOM and E-DOM in situ. In this work, a new fluorescence ratio method was developed and applied to in situ examine the impacts of polystyrene (PS) MPs (50, 500 nm, and 5 μm) on the I-DOM and E-DOM released by Skeletonema costatum (S. costatum). The experimental results indicated that the detection limits were 0.06 mg L−1, with the respective minimum detectable proportions being 2% for both E-DOM and I-DOM. The suppressive effects of 10–50 mg L−1 of 50 and 500 nm PS MPs on the cell proliferation of S. costatum and the E-DOM secretion were most pronounced on day 6. And the rates of suppression of E-DOM secretion were 10.1%–18.2% and 4.2%–13.9%, respectively. The exposure of algal cells to 50 mg L−1 of 50 and 500 nm PS MPs led to cell rupture and the leakage of I-DOM on day 6. This suggests that the developed method in the laboratory could offer a promising approach for studying the generation of E-DOM and I-DOM in situ, as well as their environmental behaviors affected by MPs.
期刊介绍:
Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health.
Subject areas include, but are not limited to:
• Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies;
• Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change;
• Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects;
• Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects;
• Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest;
• New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.