{"title":"使用外周 CD3+γδ+Vδ2+ T 淋巴细胞结合 ALBI 评分预测晚期肝细胞癌患者的免疫治疗反应:一项回顾性研究。","authors":"Shuhan Zhang, Luyang Li, Chengli Liu, Meng Pu, Yingbo Ma, Tao Zhang, Jiaqi Chai, Haoming Li, Jun Yang, Meishan Chen, Linghong Kong, Tian Xia","doi":"10.1007/s00432-024-05896-y","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Currently, there is a lack of effective indicators for predicting the efficacy of immunotherapy in patients with advanced hepatocellular carcinoma (HCC). This study aimed to investigate the expression and prognostic value of peripheral T lymphocyte subsets in advanced HCC.</p><p><strong>Methods: </strong>Patients with advanced HCC who were treated with immune checkpoint inhibitors (ICIs) from December 2021 to December 2023 were included in the study. Flow cytometry was used to detect lymphocyte subsets before treatment. The patients were divided into disease control (DC) and nondisease control (nDC) groups based on treatment efficacy. Relationships between the clinical characteristics/peripheral T lymphocytes and immunotherapy efficacy were analyzed. The effectiveness of peripheral T lymphocyte subsets in predicting immunotherapy efficacy for patients with advanced HCC was analyzed using receiver operating characteristic (ROC) curves.</p><p><strong>Results: </strong>A total of 40 eligible patients were included in this study. Non-DC was significantly associated with higher albumin-bilirubin (ALBI) scores. The percentages of γδ<sup>+</sup>Vδ2<sup>+</sup>PD1<sup>+</sup> T cells and γδ<sup>+</sup>Vδ2<sup>+</sup>Tim3<sup>+</sup> T cells were greater in the nDC group than in the DC group. Multivariable regression analysis revealed that the ALBI score and T lymphocytes expressing γδ<sup>+</sup>Vδ2<sup>+</sup>PD1<sup>+</sup> and γδ<sup>+</sup>Vδ2<sup>+</sup>Tim3<sup>+</sup> were founded to be independent influencing factors. The area under the ROC curve (AUC) values for these combinations was 0.944 (95% CI, 0.882 ~ 1.000).</p><p><strong>Conclusions: </strong>The calculation of the ALBI score and determination of the percentages CD3<sup>+</sup>γδ<sup>+</sup>Vδ2<sup>+</sup>PD1<sup>+</sup> T lymphocytes and CD3<sup>+</sup>γδ<sup>+</sup>Vδ2<sup>+</sup>Tim3<sup>+</sup> T lymphocytes in the peripheral blood of patients with advanced HCC are helpful for predicting the patients' responses to ICIs, helping to screen patients who may clinically benefit from immunotherapy. RETROSPECTIVELY REGISTERED: number: ChiCTR2400080409, date of registration: 2024-01-29.</p>","PeriodicalId":15118,"journal":{"name":"Journal of Cancer Research and Clinical Oncology","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11272815/pdf/","citationCount":"0","resultStr":"{\"title\":\"The use of peripheral CD3<sup>+</sup>γδ<sup>+</sup>Vδ2<sup>+</sup> T lymphocyte cells in combination with the ALBI score to predict immunotherapy response in patients with advanced hepatocellular carcinoma: a retrospective study.\",\"authors\":\"Shuhan Zhang, Luyang Li, Chengli Liu, Meng Pu, Yingbo Ma, Tao Zhang, Jiaqi Chai, Haoming Li, Jun Yang, Meishan Chen, Linghong Kong, Tian Xia\",\"doi\":\"10.1007/s00432-024-05896-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Currently, there is a lack of effective indicators for predicting the efficacy of immunotherapy in patients with advanced hepatocellular carcinoma (HCC). This study aimed to investigate the expression and prognostic value of peripheral T lymphocyte subsets in advanced HCC.</p><p><strong>Methods: </strong>Patients with advanced HCC who were treated with immune checkpoint inhibitors (ICIs) from December 2021 to December 2023 were included in the study. Flow cytometry was used to detect lymphocyte subsets before treatment. The patients were divided into disease control (DC) and nondisease control (nDC) groups based on treatment efficacy. Relationships between the clinical characteristics/peripheral T lymphocytes and immunotherapy efficacy were analyzed. The effectiveness of peripheral T lymphocyte subsets in predicting immunotherapy efficacy for patients with advanced HCC was analyzed using receiver operating characteristic (ROC) curves.</p><p><strong>Results: </strong>A total of 40 eligible patients were included in this study. Non-DC was significantly associated with higher albumin-bilirubin (ALBI) scores. The percentages of γδ<sup>+</sup>Vδ2<sup>+</sup>PD1<sup>+</sup> T cells and γδ<sup>+</sup>Vδ2<sup>+</sup>Tim3<sup>+</sup> T cells were greater in the nDC group than in the DC group. Multivariable regression analysis revealed that the ALBI score and T lymphocytes expressing γδ<sup>+</sup>Vδ2<sup>+</sup>PD1<sup>+</sup> and γδ<sup>+</sup>Vδ2<sup>+</sup>Tim3<sup>+</sup> were founded to be independent influencing factors. The area under the ROC curve (AUC) values for these combinations was 0.944 (95% CI, 0.882 ~ 1.000).</p><p><strong>Conclusions: </strong>The calculation of the ALBI score and determination of the percentages CD3<sup>+</sup>γδ<sup>+</sup>Vδ2<sup>+</sup>PD1<sup>+</sup> T lymphocytes and CD3<sup>+</sup>γδ<sup>+</sup>Vδ2<sup>+</sup>Tim3<sup>+</sup> T lymphocytes in the peripheral blood of patients with advanced HCC are helpful for predicting the patients' responses to ICIs, helping to screen patients who may clinically benefit from immunotherapy. RETROSPECTIVELY REGISTERED: number: ChiCTR2400080409, date of registration: 2024-01-29.</p>\",\"PeriodicalId\":15118,\"journal\":{\"name\":\"Journal of Cancer Research and Clinical Oncology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-07-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11272815/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Cancer Research and Clinical Oncology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s00432-024-05896-y\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cancer Research and Clinical Oncology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00432-024-05896-y","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景:目前,尚缺乏有效的指标来预测晚期肝细胞癌(HCC)患者免疫疗法的疗效。本研究旨在探讨外周T淋巴细胞亚群在晚期HCC中的表达和预后价值:研究纳入了2021年12月至2023年12月期间接受免疫检查点抑制剂(ICIs)治疗的晚期HCC患者。在治疗前使用流式细胞术检测淋巴细胞亚群。根据疗效将患者分为疾病控制组(DC)和非疾病控制组(nDC)。分析了临床特征/外周T淋巴细胞与免疫疗法疗效之间的关系。利用接收者操作特征曲线(ROC)分析了外周T淋巴细胞亚群在预测晚期HCC患者免疫治疗疗效方面的有效性:本研究共纳入了40名符合条件的患者。非 DC 与较高的白蛋白-胆红素(ALBI)评分明显相关。nDC组中γδ+Vδ2+PD1+ T细胞和γδ+Vδ2+Tim3+ T细胞的百分比高于DC组。多变量回归分析显示,ALBI评分和表达γδ+Vδ2+PD1+和γδ+Vδ2+Tim3+的T淋巴细胞是独立的影响因素。这些组合的 ROC 曲线下面积(AUC)值为 0.944(95% CI,0.882 ~ 1.000):结论:计算 ALBI 评分和确定晚期 HCC 患者外周血中 CD3+γδ+Vδ2+PD1+ T 淋巴细胞和 CD3+γδ+Vδ2+Tim3+ T 淋巴细胞的百分比有助于预测患者对 ICIs 的反应,有助于筛选临床上可能从免疫疗法中获益的患者。回溯注册:编号:ChiCTR2400080409,注册日期:2024-01-29。
The use of peripheral CD3+γδ+Vδ2+ T lymphocyte cells in combination with the ALBI score to predict immunotherapy response in patients with advanced hepatocellular carcinoma: a retrospective study.
Background: Currently, there is a lack of effective indicators for predicting the efficacy of immunotherapy in patients with advanced hepatocellular carcinoma (HCC). This study aimed to investigate the expression and prognostic value of peripheral T lymphocyte subsets in advanced HCC.
Methods: Patients with advanced HCC who were treated with immune checkpoint inhibitors (ICIs) from December 2021 to December 2023 were included in the study. Flow cytometry was used to detect lymphocyte subsets before treatment. The patients were divided into disease control (DC) and nondisease control (nDC) groups based on treatment efficacy. Relationships between the clinical characteristics/peripheral T lymphocytes and immunotherapy efficacy were analyzed. The effectiveness of peripheral T lymphocyte subsets in predicting immunotherapy efficacy for patients with advanced HCC was analyzed using receiver operating characteristic (ROC) curves.
Results: A total of 40 eligible patients were included in this study. Non-DC was significantly associated with higher albumin-bilirubin (ALBI) scores. The percentages of γδ+Vδ2+PD1+ T cells and γδ+Vδ2+Tim3+ T cells were greater in the nDC group than in the DC group. Multivariable regression analysis revealed that the ALBI score and T lymphocytes expressing γδ+Vδ2+PD1+ and γδ+Vδ2+Tim3+ were founded to be independent influencing factors. The area under the ROC curve (AUC) values for these combinations was 0.944 (95% CI, 0.882 ~ 1.000).
Conclusions: The calculation of the ALBI score and determination of the percentages CD3+γδ+Vδ2+PD1+ T lymphocytes and CD3+γδ+Vδ2+Tim3+ T lymphocytes in the peripheral blood of patients with advanced HCC are helpful for predicting the patients' responses to ICIs, helping to screen patients who may clinically benefit from immunotherapy. RETROSPECTIVELY REGISTERED: number: ChiCTR2400080409, date of registration: 2024-01-29.
期刊介绍:
The "Journal of Cancer Research and Clinical Oncology" publishes significant and up-to-date articles within the fields of experimental and clinical oncology. The journal, which is chiefly devoted to Original papers, also includes Reviews as well as Editorials and Guest editorials on current, controversial topics. The section Letters to the editors provides a forum for a rapid exchange of comments and information concerning previously published papers and topics of current interest. Meeting reports provide current information on the latest results presented at important congresses.
The following fields are covered: carcinogenesis - etiology, mechanisms; molecular biology; recent developments in tumor therapy; general diagnosis; laboratory diagnosis; diagnostic and experimental pathology; oncologic surgery; and epidemiology.