Proxitome 图谱揭示了激活 NLR 介导的免疫的保守 SGT1-NSL1 信号模块。

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-07-25 DOI:10.1016/j.molp.2024.07.010
Dingliang Zhang, Xinxin Yang, Zhiyan Wen, Zhen Li, Xinyu Zhang, Chenchen Zhong, Jiajie She, Qianshen Zhang, He Zhang, Wenli Li, Xiaoyun Zhao, Mingliang Xu, Zhen Su, Dawei Li, Savithramma P Dinesh-Kumar, Yongliang Zhang
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引用次数: 0

摘要

SGT1 是一种高度保守的真核蛋白,在动物和植物的生长、发育和免疫中发挥着重要作用。虽然已经发现了一些 SGT1 的相互作用者,但 SGT1 的分子调控网络仍不清楚。SGT1 是一种辅助伴侣蛋白,可稳定核苷酸结合富亮氨酸重复(NLR)类免疫受体等蛋白质复合物,从而积极调节植物免疫。还发现 SGT1 与 SKP1-Cullin-F-box (SCF) E3 泛素连接酶复合物有关。然而,SGT1 是否以免疫抑制因子为靶标来协调植物免疫激活仍是一个未知数。在这里,我们构建了一个工具箱,用于基于 TurboID 和 split-TurboID 的近距离标记(PL)测定。我们利用PL工具箱探索了免疫激活前后的SGT1相互作用组。我们发现的全面的 SGT1 相互作用组网络突显了从与植物发育相关的蛋白质到与植物免疫反应相关的蛋白质的动态转变。SGT1 与坏死性斑点病变 1(NSL1)相互作用,NSL1 在 NLR 介导的对烟草花叶病毒(TMV)的应答过程中,通过干扰非致病相关基因表达者 1(NPR1)的核胞浆转运,对水杨酸(SA)介导的防御起负性调节作用。SGT1 可促进 NSL1 的 SCF 依赖性降解,从而促进免疫激活,而水杨酸诱导蛋白激酶(SIPK)介导的 SGT1 磷酸化可进一步加强这一过程。除 N NLR 外,NSL1 还在其他几种 NLR 介导的免疫中发挥作用。我们的研究揭示了 SGT1 的调控格局,并揭示了一个协调植物先天免疫的新型 SGT1-NSL1 信号模块。
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Proxitome profiling reveals a conserved SGT1-NSL1 signaling module that activates NLR-mediated immunity.

Suppressor of G2 allele of skp1 (SGT1) is a highly conserved eukaryotic protein that plays a vital role in growth, development, and immunity in both animals and plants. Although some SGT1 interactors have been identified, the molecular regulatory network of SGT1 remains unclear. SGT1 serves as a co-chaperone to stabilize protein complexes such as the nucleotide-binding leucine-rich repeat (NLR) class of immune receptors, thereby positively regulating plant immunity. SGT1 has also been found to be associated with the SKP1-Cullin-F-box (SCF) E3 ubiquitin ligase complex. However, whether SGT1 targets immune repressors to coordinate plant immune activation remains elusive. In this study, we constructed a toolbox for TurboID- and split-TurboID-based proximity labeling (PL) assays in Nicotiana benthamiana and used the PL toolbox to explore the SGT1 interactome during pre- and post-immune activation. The comprehensive SGT1 interactome network we identified highlights a dynamic shift from proteins associated with plant development to those linked with plant immune responses. We found that SGT1 interacts with Necrotic Spotted Lesion 1 (NSL1), which negatively regulates salicylic acid-mediated defense by interfering with the nucleocytoplasmic trafficking of non-expressor of pathogenesis-related genes 1 (NPR1) during N NLR-mediated response to tobacco mosaic virus. SGT1 promotes the SCF-dependent degradation of NSL1 to facilitate immune activation, while salicylate-induced protein kinase-mediated phosphorylation of SGT1 further potentiates this process. Besides N NLR, NSL1 also functions in several other NLR-mediated immunity. Collectively, our study unveils the regulatory landscape of SGT1 and reveals a novel SGT1-NSL1 signaling module that orchestrates plant innate immunity.

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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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