评估花、叶和茎提取物的植物化学成分、抗氧化、抗溶血、抗炎和抗癌潜力。

Zubaria Tul Ain, Iram Fatima, Sana Naseer, Sobia Kanwal, Tariq Mahmood
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引用次数: 0

摘要

目的:评估蔷薇花、叶和茎提取物的植物化学成分和体外生物学潜力:评估蔷薇花、叶和茎提取物的植物化学成分和体外生物学潜力:方法:确认了二十种次级代谢产物的存在,然后用分光光度法对酚类和类黄酮的含量进行了定量。采用傅立叶变换红外光谱法确定功能基团,并利用 2,2-二苯基-1-苦基肼清除活性、总抗氧化能力和总还原力检测抗氧化潜力。利用人体红细胞评估抗溶血活性,并检测五种细菌菌株,以确定植物提取物的抗菌潜力。萝卜种子被用来进行植物毒性试验,盐水虾和 PC3 细胞系被用来评估细胞毒性潜力:结果:蔷薇花甲醇提取物(RAFM)中酚含量最高[(151.635 ± 0.005)没食子酸当量毫克/克],氯仿叶提取物(RALC)中黄酮含量最高[(108.228 ± 0.004)槲皮素当量毫克/克]。傅立叶变换红外光谱分析显示了多种官能团的存在。抗氧化试验表明,甲醇茎提取物(RASM)的 DPPH 清除活性最高[IC50 (23.5 ± 0.6) μg/mL],RAFM 的总抗氧化能力最高[(265.1 ± 0.9) μg/mL],叶提取物(RALM)的还原电位最高[(209.9 ± 0.6) μg/mL]。在 RAFM 中记录到最高的抗溶血活性[(90.0 ± 0.5)μg/mL],在 RASM 中记录到最高的盐水虾细胞毒性潜能[(52.3 ± 0.3)μg/mL]。RALM 对金黄色葡萄球菌的抗菌活性最高[(21.1 ± 0.5)毫米抑菌区]。最后,甲醇提取物的抗炎和抗癌活性抑制率低于 50%。结论:我们的研究结果将有助于设计药物治疗方案,因此建议开展更多研究,以分离和鉴定与蔷薇生物活性相关的化合物。
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Assessment of phytochemicals, antioxidant, anti-hemolytic, anti-inflammatory and anti-cancer potential of flowers, leaves and stem extracts of.

Objective: To evaluate phytochemicals and in vitro biological potential of flowers, leaves and stem extracts of Rosa arvensis.

Methods: Presence of twenty secondary metabolites was confirmed and then phenolic and flavonoid contents were quantified spectrophotometrically. Fourier Transform Infrared spectroscopy was conducted to ascertain functional groups and antioxidant potential was examined using 2,2-diphenyl-1-picrylhydrazyl scavenging activity, total antioxidant capacity and total reducing power assays. Human erythrocytes were used to assess anti-hemolytic activity and five bacterial strains were examined to determine antibacterial potential of plant extracts. Radish seeds were used to perform phytotoxic activity and cytotoxic potential was evaluated via brine shrimps and PC3 cell lines.

Results: Highest phenolic contents were detected in the methanolic extract of Rosa arvensis flower (RAFM) [(151.635 ± 0.005) gallic acid equivalent mg/g] and highest flavonoid contents in the chloroform leaf extract (RALC) [(108.228 ± 0.004) quercetin equivalent mg/g]. Fourier-transform infrared spectroscopy analysis showed the presence of wide range of functional groups. The antioxidant assays indicated highest DPPH scavenging activity [IC50 (23.5 ± 0.6) μg/mL] in the methanolic stem extract (RASM), highest total antioxidant capacity [(265.1 ± 0.9) μg/mL] in RAFM and highest reducing potential [(209.9 ± 0.6) μg/mL] in leaf extract (RALM). Highest anti-hemolytic activity [(90.0 ± 0.5) μg/mL] was recorded in RAFM and brine shrimp cytotoxicity potential [(52.3 ± 0.3) μg/mL] in RASM. The antimicrobial activity was detected highest [(21.1 ± 0.5) mm inhibition zones] in RALM against Streptococcus aureus. In the end, anti-inflammatory and anti-cancer activity results depicted less than 50 % inhibition in the methanolic extracts. CONCLUSIONS: Our findings will be helpful in designing pharmaceutical regimens and therefore, more studies can be recommended to isolate and characterize compounds associated with the biological activities of Rosa arvensis.

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