脉冲电场辅助从节肢动物生物质中提取蛋白质和藻蓝蛋白:动力学研究

IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food and Bioproducts Processing Pub Date : 2024-07-20 DOI:10.1016/j.fbp.2024.07.012
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引用次数: 0

摘要

这项工作的目的是研究在脉冲电场(PEF)辅助下从(螺旋藻)生物质中提取蛋白质和 C-花青素的动力学。将未经处理和经 PEF 处理(7.8 kV/cm,0-137.6 kJ/kg)的新鲜生物质悬浮于水中,在 30-50 °C 下培养 24 小时。蛋白质和 C-phycocyanin(C-花青素)的恢复动力学表现为曲线行为。处理比能量和培养温度提高到 40 °C,萃取速度明显加快。在 40 °C 下培养前进行 137.6 kJ/kg 的处理可最大限度地缩短蛋白质特征培养时间(2.3 小时,而在 30 °C 和 40 °C 下未经处理的样品分别为 14.4 小时和 10.4 小时)。在 50 °C 时,由于蛋白水解酶失活,提取率明显下降。在 40 °C 时,较高的处理比能量可提高提取物中 C-phycocyanin 的纯度。137.6 kJ/kg 的 PEF 处理和 40 °C 培养 3 小时可使 C-花青素提取物纯度系数最大化(1.14 对未处理样品的 0.66)。
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Pulsed Electric Fields assisted extraction of proteins and phycocyanin from Arthrospira platensis biomass: A kinetic study

The objective of this work was the investigation of the kinetics of protein and C-phycocyanin extraction from Arthrospira platensis (Spirulina) biomass assisted by Pulsed Electric Fields (PEF). Fresh, untreated and PEF-treated (7.8 kV/cm, 0–137.6 kJ/kg) biomass was suspended in water and incubated at 30–50 °C for up to 24 h. Protein and C-phycocyanin recovery kinetics exhibited a sigmoidal behavior. The increase in treatment specific energy and incubation temperature up to 40 °C led to significant extraction acceleration. A treatment of 137.6 kJ/kg prior to incubation at 40 °C led to the minimization of protein characteristic incubation time (2.3 h vs. 14.4 h and 10.4 h of untreated sample at 30 and 40 °C, respectively). At 50 °C a significant reduction of the extraction yield was observed, due to the inactivation of the proteolytic enzymes. Higher treatment specific energy led to increased C-phycocyanin purity of the extracts at 40 °C. The maximization of the C-phycocyanin extract purity factor (1.14 vs 0.66 for untreated samples) was achieved with a PEF treatment at 137.6 kJ/kg and incubation at 40 °C for 3 h.

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来源期刊
Food and Bioproducts Processing
Food and Bioproducts Processing 工程技术-工程:化工
CiteScore
9.70
自引率
4.30%
发文量
115
审稿时长
24 days
期刊介绍: Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.
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