A. D. Novikov, I. P. Tokmakova, A. A. Samarin, K. V. Lavrov, A. S. Yanenko
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引用次数: 0
摘要
摘要 这是首次报道分离出Rhodococcus aetherivorans特异性噬菌体,以及将无脊椎动物Hyalophora cecropia和Eisenia fetida作为筛选能在Rhodococcus物种上生长的噬菌体的对象的适用性。分离出的一些噬菌体能够在R.ruber和R.qingshengii上繁殖。研究人员开发了一种在 R. aetherivorans 的液体培养物中繁殖噬菌体的有效程序。所发现的噬菌体可用于开发针对罗氏球菌菌株(包括具有重要工业价值的菌株)的高效遗传工具。
Search for Bacteriophages Specific against Members of the Genus Rhodococcus
Abstract
This is the first report on the isolation of Rhodococcus aetherivorans-specific bacteriophages and of applicability of such invertebrates as Hyalophora cecropia and Eisenia fetida as objects for screening the phages capable of growth on Rhodococcus species. Some of the isolated phages were capable of reproduction on R. ruber and R. qingshengii. An efficient procedure for bacteriophage reproduction in the liquid culture of R. aetherivorans was developed. The revealed bacteriophages may be used for development of efficient genetic tools for Rhodococcus strains, including the industrially significant ones.
期刊介绍:
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.