Paul F. Cullen, William J. Gammerdinger, Shannan J. Ho Sui, Arpan Guha Mazumder, Daniel Sun
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引用次数: 0
摘要
封面插图:通过显微切割从 GFAP-Cre+ x Rpl22HA/HA 小鼠体内分离出的视网膜星形胶质细胞(绿色)的共焦图像显示,HA 标记的核糖体(红色)遍布整个细胞,包括内膜过程,这表明通过免疫沉淀可以分离出整个细胞中与核糖体相关的 mRNA。该图像还显示了我们的显微切割方法对星形胶质细胞形态的良好保存。细胞核用蓝色 DAPI 标记。(见 Cullen, P. 等人,https://doi.org/10.1002/glia.24571)
Cover Illustration: Confocal image of retinal astrocyte (green) isolated by microdissection from GFAP-Cre+ x Rpl22HA/HA mouse showing HA-tagged ribosomes (red) present throughout the cell, including within endfeet processes, indicating that ribosome associated mRNA throughout the cell can be isolated by immunoprecipitation. The image also indicates how well astrocyte morphology are preserved in our microdissection method. Nuclei are labeled with DAPI in blue. (See Cullen, P., et al, https://doi.org/10.1002/glia.24571)
期刊介绍:
GLIA is a peer-reviewed journal, which publishes articles dealing with all aspects of glial structure and function. This includes all aspects of glial cell biology in health and disease.