阴道分离物中微生物的流行率和易感性

Christian González-Reyes, Ahily Guadalupe Rosas-Partida, Lesset del Consuelo Ramos-Ramírez, Jackeline Lizzeta Arvizu-Gómez, Nadia Roxana Martínez-Rubio, Eduardo Mendeleev Becerra-Verdín, Angélica Nallelhy Rodríguez-Ocampo
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摘要

通过对生殖器分泌物进行微生物分析,可以分离和鉴定导致阴道感染的微生物。这项研究的目的是分离和鉴定导致阴道感染的微生物,并分析抗菌药的敏感性。研究人员分析了 210 份样本,这些样本来自临床诊断为阴道感染并前往微生物学服务机构就诊的妇女。对样本进行了微生物分析和革兰氏染色,并在特定的培养基和穆勒-辛顿培养基中进行播种,以进行药敏试验。共鉴定出 115 种微生物,其中 57% 为细菌,最常见的是阴道加德纳菌,占 38.5%。43%的分离物为酵母菌,主要菌种为白色念珠菌,占 78%。另一方面,在对各种抗生素的敏感性测试中,阴道加德纳菌对庆大霉素和奈替米星显示出 100%的抗药性。氨苄西林、诺氟沙星和磺胺甲恶唑/三甲氧苄青霉素的敏感性超过 80%。在抗真菌药敏试验中,白色念珠菌对硝司他丁的抗药性为 100%,对氟康唑和酮康唑的敏感性分别为 90%至 100%。在分离出的菌种中,阴道加德纳菌和白色念珠菌是阴道感染中最常见的菌种;此外,这两种微生物的敏感性也各不相同。
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Prevalence and susceptibility of microorganisms in vaginal isolates
Microbiological analysis of genital secretion allows isolate and identify the microorganisms responsible for infection in the vaginal canal. The objective of this research was to isolate and identify the microorganisms that cause vaginal infection and analyze antimicrobial susceptibility. 210 samples from women with a clinical diagnosis of vaginal infection who attended the microbiology service were analyzed. Microbial analysis, Gram staining was performed, and they were seeded in specific culture media, as well as in Mueller-Hinton for sensitivity tests. 115 microorganisms were identified, 57% of the isolated correspond to bacterial species, the most frequent was Gardnerella vaginalis with 38.5%. 43% of the isolated were yeasts, the predominant species was Candida albicans with 78%. On the other hand, testing for susceptibility to various antibiotics, Gardnerella vaginalis showed 100% resistance to gentamicin and netilmicin. Ampicillin, norfloxacin and sulfamethoxazole/trimethoprim showed sensitivity greater than 80%. In antifungal susceptibility tests, Candida albicans showed 100% resistance to nystatin and 90 to 100% sensitivity to fluconazole and ketoconazole, respectively. Of the isolated species, Gardnerella vaginalis and Candida albicans were the most frequent in vaginal infections; in addition, both microorganisms presented a variable susceptibility
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