结合磁共振成像、组织学和免疫组织化学方法对猕猴的脑室(LC)进行渲染,可以区分三个不同的脑室亚区。

IF 2.7 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of chemical neuroanatomy Pub Date : 2024-07-29 DOI:10.1016/j.jchemneu.2024.102449
Irina T. Sinakevitch , Kelsey E. McDermott , Daniel T. Gray , Carol A. Barnes
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引用次数: 0

摘要

神经元的去甲肾上腺素能轴突遍布多个脑区,包括新皮层、皮层下区和脊髓。众所周知,认知的许多方面都依赖于去甲肾上腺素能系统,有人认为,该系统的功能障碍可能在与正常衰老和神经退行性疾病相关的认知能力下降中起着核心作用。虽然许多物种的低密度脂蛋白胆固醇的基本解剖和生化特征已得到研究,但目前还没有关于低密度脂蛋白胆固醇在整个生命周期中的结构和功能的详细描述。这其中就包括猕猴,猕猴是人类大脑功能的重要模型,因为它们在大脑结构和行为能力方面有着惊人的相似之处。在本研究中,我们介绍了一种结合结构磁共振成像、Nissl 和免疫荧光组织学的方法,从单个猴子的三维角度重建整个猕猴 LC 核。利用这些综合方法,确定了 LC 的标准化体积,并将酪氨酸羟化酶阳性神经元的高分辨率共聚焦图像映射到该体积中。LC 的这一详细表征为三个不同的亚核提出了定义,包括一个内侧区和一个外侧区(分别基于相对于中央灰质的内部或外部位置),以及一个密集区(由内侧区内密集的神经元定义)。这样就能首次独立估算每个 LC 亚核的体积并计算细胞密度。这种方法的结合应能精确描述 LC 的特征,并有可能对具有独特分子特征的其他核进行同样的分析。
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A combined MRI, histological and immunohistochemical rendering of the rhesus macaque locus coeruleus (LC) enables the differentiation of three distinct LC subcompartments

Locus coeruleus (LC) neurons send their noradrenergic axons across multiple brain regions, including neocortex, subcortical regions, and spinal cord. Many aspects of cognition are known to be dependent on the noradrenergic system, and it has been suggested that dysfunction in this system may play central roles in cognitive decline associated with both normative aging and neurodegenerative disease. While basic anatomical and biochemical features of the LC have been examined in many species, detailed characterizations of the structure and function of the LC across the lifespan are not currently available. This includes the rhesus macaque, which is an important model of human brain function because of their striking similarities in brain architecture and behavioral capacities. In the present study, we describe a method to combine structural MRI, Nissl, and immunofluorescent histology from individual monkeys to reconstruct, in 3 dimensions, the entire macaque LC nucleus. Using these combined methods, a standardized volume of the LC was determined, and high-resolution confocal images of tyrosine hydroxylase-positive neurons were mapped into this volume. This detailed representation of the LC allows definitions to be proposed for three distinct subnuclei, including a medial region and a lateral region (based on location with respect to the central gray, inside or outside, respectively), and a compact region (defined by densely packed neurons within the medial compartment). This enabled the volume to be estimated and cell density to be calculated independently in each LC subnucleus for the first time. This combination of methods should allow precise characterization of the LC and has the potential to do the same for other nuclei with distinct molecular features.

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来源期刊
Journal of chemical neuroanatomy
Journal of chemical neuroanatomy 医学-神经科学
CiteScore
4.50
自引率
3.60%
发文量
87
审稿时长
62 days
期刊介绍: The Journal of Chemical Neuroanatomy publishes scientific reports relating the functional and biochemical aspects of the nervous system with its microanatomical organization. The scope of the journal concentrates on reports which combine microanatomical, biochemical, pharmacological and behavioural approaches. Papers should offer original data correlating the morphology of the nervous system (the brain and spinal cord in particular) with its biochemistry. The Journal of Chemical Neuroanatomy is particularly interested in publishing important studies performed with up-to-date methodology utilizing sensitive chemical microassays, hybridoma technology, immunocytochemistry, in situ hybridization and receptor radioautography, to name a few examples. The Journal of Chemical Neuroanatomy is the natural vehicle for integrated studies utilizing these approaches. The articles will be selected by the editorial board and invited reviewers on the basis of their excellence and potential contribution to this field of neurosciences. Both in vivo and in vitro integrated studies in chemical neuroanatomy are appropriate subjects of interest to the journal. These studies should relate only to vertebrate species with particular emphasis on the mammalian and primate nervous systems.
期刊最新文献
Editorial Board Retraction notice to “Astrocyte response to melatonin treatment in rats under high-carbohydrate high-fat diet” [J. Chem. Neuroanat. 136 (2024) 102389] Retraction notice to “Coenzyme Q10 attenuates neurodegeneration in the cerebellum induced by chronic exposure to tramadol” [J. Chem. Neuroanat. 135 (2024) 102367] Retraction notice to “Maternal diabetes-induced alterations in the expression of brain-derived neurotrophic factor in the developing rat hippocampus” [J. Chem. Neuroanat. 114(2021) 101946] Retraction notice to “Aqueous leaf extract of Phyllanthus amarus protects against oxidative stress and misfiring of dopaminergic neurons in Paraquat-induced Parkinson's disease-like model of adult Wistar rats” [J. Chem. Neuroanat. 135 (2024) 102365]
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