Tooth Discoloration Induced by Calcium Silicate-based Sealers

Aims: The propose of this study was to evaluate, using spectrophotometric analysis, the change in tooth color induced by different calcium silicate-based sealers over the period of 24 hours and 30 days. Methodology: Sixty bovine teeth were sectioned in enamel-dentin blocks. They were filled with AH Plus Bioceramic, AH Plus Jet, Bio-C Sealer and BioRoot RCS and sealed with composite. Triple antibiotic paste and unfilled samples were positive and negative control groups, respectively. Specimens were stored in separate flasks immersed in tap water at 37ºC with ambient light block out. Spectrophotometric analysis for color assessment was performed at different periods, before filling, 24 hours, and 30 days after filling. Luminosity (L) and color change ( ) were calculated. The statistical analysis was performed by using ANOVA and Tukey’s test (P < 0.05). Results: All calcium silicate-based sealers exhibited high color alteration after the evaluated period of 30 days and showed statistically significant difference values of  in relation with the negative control in the period of 30 days (P < 0.05). Luminosity was not affected in comparison with negative control, in both evaluated periods (P < 0.05). Conclusion: All tested sealers showed certain levels of tooth discoloration. Calcium silicate-based sealers (AH Plus Bioceramic, Bio-C Sealer, and BioRoot RCS) presented discoloration exceeding acceptable values within a 30-day follow-up. Notwithstanding, these alterations were imperceptible to the human eye.