利用有机电化学晶体管对 PEDOT:PSS 进行功能化,以实现基于适配体的 IL6 检测

Bernhard Burtscher, Chiara Diacci, Anatolii Makhinia, Marios Savvakis, Erik O. Gabrielsson, Lothar Veith, Xianjie Liu, Xenofon Strakosas, Daniel T. Simon
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摘要

在此,我们提出了一种基于聚(乙烯二氧噻吩)-聚(苯乙烯磺酸)(PEDOT:PSS)的有机电化学晶体管(OECTs)的功能化策略,用于感测炎症细胞因子白细胞介素 6(IL6)。为此,我们使用重氮化学方法将 4-aminobenzoic acid 与 PSS 上的磺酸盐分子耦合,后者可作为适配体或其他识别元素(如荧光或氧化还原探针)的锚。我们使用商业丝网印刷 PEDOT:PSS 配方研究了这种方法,同时还研究了 PEDOT 与 PSS 的比例以及其他 PEDOT:PSS 配方中交联剂用量的影响。对于丝网印刷的 OECTs,可以区分缓冲溶液中的 IL6 和牛血清白蛋白 (BSA),并能在牛血浆中检测出纳摩尔范围内的 IL6。此外,与 OECTs 使用的 "标准 "溶液相比,PEDOT:PSS 含量更高的 PEDOT:PSS 配方的功能化与频率相关测量相结合,显示出检测低于 100 pM 的 IL6 浓度的潜力。
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Functionalization of PEDOT:PSS for aptamer-based sensing of IL6 using organic electrochemical transistors
Here we propose a strategy to functionalize poly(ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) based organic electrochemical transistors (OECTs) for sensing the inflammatory cytokine interleukin 6 (IL6). For this aim we use diazonium chemistry to couple 4-aminobenzoic acid to sulfonate moieties on the PSS, which can act as anchors for aptamers or other recognition elements (e.g., fluorescent, or redox probes). We investigated this approach with a commercial screen-printable PEDOT:PSS formulation but also studied the effect of PEDOT to PSS ratio as well as the amount of crosslinker in other PEDOT:PSS formulations. For screen printed OECTs, it was possible to distinguish between IL6 and bovine serum albumin (BSA) in buffer solution and detect IL6 when added in bovine plasma in the nanomolar range. Furthermore, functionalization of PEDOT:PSS formulations with higher PSS content (compared to the “standard” solutions used for OECTs) combined with frequency dependent measurements showed the potential to detect IL6 concentrations below 100 pM.
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