利用超声辅助深共晶溶剂萃取法从 Phyllostachys glauca McClure 叶中提取的五种重要酚类物质

Jianqiang Hei, Jianjun Wang, Jin Wang, Daoqi Zhang, Wenting Song, Hang Xun, Xuefeng Guo, Xi Yao
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摘要

Phyllostachys glauca McClure 叶子(PML)作为一种经济的天然产品材料,含有丰富的酚类物质,尤其是黄酮类物质,具有显著的生物活性,因此被广泛应用于食品、医药和化妆品行业。为了研究 PML 中的重要酚类物质,研究人员建立了一种高效、低毒的超声辅助深共晶溶剂萃取法,用于从 PML 和其他 17 种竹子的竹叶中提取酚类物质。采用超高效液相色谱法(UPLC)测定了 18 种竹叶中的酚含量。结果发现,PML中含有新绿原酸、绿原酸、隐绿原酸、异绿原酸和荭草苷,含量分别为0.793 ± 00.17 mg/g、0.491 ± 0.0081 mg/g、0.485 ± 0.0043 mg/g、0.465 ± 0.0021 mg/g和0.044 ± 0.0005 mg/g,总含量为2.278 mg/g。这些含量明显高于其他 17 种竹子叶片中的含量。此外,在 15 种竹子的叶片中同时发现了重要的酚类、新绿原酸、绿原酸和隐绿原酸,尤其是在 PML 中。因此,PML 因其丰富的酚类物质可被视为具有相当应用价值的天然产品材料,在利用新绿原酸、绿原酸和隐绿原酸作为资源方面具有重要意义。
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Five Significant Phenols from Phyllostachys glauca McClure Leaves Extracted Using Ultrasound-Assisted Deep Eutectic Solvent Extraction
Phyllostachys glauca McClure leaves (PML), as economical natural product material, contain abundant phenols, particularly flavonoids, with significant biological activities that contribute to their widespread applications in food, pharmaceutical, and cosmetic industries. To study the significant phenols in PML, an ultrasound-assisted deep eutectic solvent extraction method with high efficiency and low toxicity was established for extracting the phenols from PML and the bamboo leaves of 17 other bamboo species. Using the Ultra Performance Liquid Chromatography (UPLC) method, the content of phenols in the leaves of the 18 bamboo species was determined. PML were found to contain neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isoorientin, and orientin at contents of 0.793 ± 00.17 mg/g, 0.491 ± 0.0081 mg/g, 0.485 ± 0.0043 mg/g, 0.465 ± 0.0021 mg/g, and 0.044 ± 0.0005 mg/g, respectively, with a total content of 2.278 mg/g. These contents were significantly higher than those found in the leaves of 17 other bamboo species. Additionally, the significant phenols, neochlorogenic acid, chlorogenic acid, and cryptochlorogenic acid were found simultaneously in the leaves of 15 bamboo species, especially in PML. Therefore, PML can be viewed as the natural product material with considerable application values, owing to its abundant phenols, and can exhibit its importance in utilizing neochlorogenic acid, chlorogenic acid, and cryptochlorogenic acid as resources.
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