用紫外法测定合成果冻并验证雷米普利

SK Umeraazmi, B. Krishna Reddy, K. Raja Sekhar Reddy, R. Ramesh, S. Jayasree, S. Sandeep, B. Thangabalan
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引用次数: 0

摘要

Chalcone 是一种 α、β- 不饱和酮,也是从天然产品中提取的多种重要生物化合物的核心,本实验通过进行鉴定测试来确定化合物中的化学成分,如 和 是本程序中使用的主要成分。使用雷米普利对合成的果冻进行了开发和验证,发现其线性范围为 10-50 µg/ml,日内精密度 % RSD 为 35.7142,日间精密度 % RSD 为 35.7277,准确度和回收率分别为 80%、100%、120%,分别为 82、95、103。特异性 % RSD 为 29.43,稳健性波长为 210 nm、211nm、212 nm,结果为 3.345、26.182、21.038,LOD - 0.8µg/ml,LOQ - 2.5 µg/ml。在给定的雷米普利样品中,查尔酮果冻的含量为 81µg/ml。
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Determination of the synthesised jelly by UV method development and validation of ramipril
Chalcone is an α,β- unsaturated ketone and central core for a variety of important biological compounds extracted from natural products and the experiment is conducted by performing identification tests to identify the chemical components in the compounds such as and are the main components used in this procedure. The synthesized jelly is developed and validated by ramipril and its linearity is found to be 10-50 µg/ml, precision % RSD of in intraday 35.7142 and in inter day 35.7277, Accuracy and recovery studies 80 %, 100%,120% are found to be 82,95,103,. Specificity % RSD is 29.43, Robustness wavelengths are taken as 210 nm, 211nm,212 nm found to be 3.345,26.182,21.038, LOD – 0.8µg/ml, LOQ - 2.5 µg/ml. The amount present in chalcone jelly in given sample of ramipril was 81µg/ml.
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