检测 KISS1 基因对萨能山羊繁殖参数的影响

D. Dinçel, Mehmed Berk Toker, I. Dogan
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摘要

KISS1 基因可激活 G 蛋白偶联受体-54(GPR54),并在 GnRH 分泌的神经内分泌调控中发挥重要作用。因此,KISS1 基因被认为与性成熟、后代发育和发情有关。KISS1 基因的 g.2124T>A 多态性被认为会影响生殖系统,到目前为止,已在印尼山羊、大马士革山羊、扎拉比山羊、巴拉迪山羊、加迪山羊、卡利格兴山羊、关宗山羊、仙农萨能山羊和波尔山羊等品种中进行了研究。这些研究通常与确定多态性频率或基因型对产仔数的影响有关。关于 KISS1 基因对黄体结构的影响,尤其是对萨能山羊的影响,目前尚未发现任何研究。本研究旨在通过PCR-RFLP方法测定KISS1基因g.2124T>A SNP的等位基因和基因型频率,并确定其基因型结构与萨能山羊(n=30)的黄体生长、最大黄体直径和交配率等繁殖参数之间的关系。结果显示,KISS1基因g.2124T>A在萨能山羊中的AA、TT和AT基因型频率分别为10.35%、55.17%和34.48%。此外,基因型结构对所调查的繁殖力特征没有显著的统计学影响。预期杂合度(He)、观察杂合度(Ho)、有效等位基因数(Ne)、固定指数(FIS)和多态性信息含量(PIC)的平均值分别为 0.3996、0.3448、1.6655、0.8629 和 0.855。哈代-温伯格方差(χ2)值为 0.5435。总之,KISS1 - g.2124T>A多态性存在基因型变异和种群规模狭窄的原因,因此有必要在具有不同基因库的较大种群中进行研究。
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Detection of the effect of the KISS1 gene on reproductive parameters in Saanen goats
The KISS1 gene, which activates G protein-coupled receptor-54 (GPR54) and plays a crucial role in the neuroendocrine regulation of GnRH secretion, is known to encode a family of neuropeptides called kisspeptins. Therefore, the KISS1 gene is thought to be associated with sexual maturity, offspring development, and estrus. The g.2124T>A polymorphism of the KISS1 gene, is believed to affect the reproductive system, has been studied in Indonesian goat breeds, Damascus, Zarabi, Baladi, Gaddi, Kaligesing, Guanzong, Xianong Saanen and Boer goat breeds until now. These studies are generally related to determining polymorphism frequencies or the effect of genotype on litter size. No studies have been found on the effect of the KISS1 gene on luteal structures, especially in Saanen goats. The study aims to determine the allele and genotype frequencies of the KISS1 gene g.2124T>A SNP by the PCR-RFLP method and define the relationship between the genotypic structures and reproductive parameters such as luteal growth, maximum corpus luteum diameter, and mating rate in Saanen goats (n=30). According to the results, the genotype frequencies in Saanen goats for the KISS1 gene g.2124T>A were 10.35%, 55.17%, and 34.48% for AA, TT and AT, respectively. Moreover, the genotypic structure did not have a statistically significant effect on investigated fertility characteristics. The average values of expected heterozygosity (He), observed heterozygosity (Ho), the effective number of alleles (Ne), fixation index values (FIS), and the polymorphism information content (PIC) were 0.3996, 0.3448, 1.6655, 0.8629 and 0.855, respectively. The Hardy-Weinberg chi-square (χ2) value was found to be 0.5435. In conclusion, it was found necessary to study the KISS1 - g.2124T>A polymorphism in larger herds with different gene pools as a reason for the existence of genotypic variation and the narrow population size.
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