葡萄 LBD 转录因子 VvLBD39 调节植物对盐和干旱胁迫的反应

IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES Environmental and Experimental Botany Pub Date : 2024-07-26 DOI:10.1016/j.envexpbot.2024.105918
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引用次数: 0

摘要

葡萄(Vitis vinifera L.)作为一种重要的落叶多年生果树,经常面临盐碱和干旱等各种非生物胁迫。侧器官边界域(LBD)蛋白是一类植物特异性转录因子,在调控植物生长和应对非生物胁迫方面发挥着关键作用。然而,人们对 LBD 转录因子在葡萄中的生物学功能仍然知之甚少。qRT-PCR分析表明,葡萄暴露于NaCl、聚乙二醇6000(PEG6000)和脱落酸(ABA)处理后,VvLBD39的表达分别下调。在葡萄胼胝体和拟南芥中过表达 VvLBD39 会导致对 PEG6000 和 NaCl 胁迫的超敏反应。此外,过表达 VvLBD39 的转基因烟草对干旱和盐胁迫的耐受性降低,对外源 ABA 也不敏感。在干旱和盐胁迫处理后,转基因烟草的叶绿素含量、根长和抗氧化酶活性均低于野生型(WT)。相反,与 WT 相比,转基因烟草的丙二醛(MDA)含量、电子电导率、过氧化氢(H2O2)含量和超氧阴离子(O2-)产生率明显升高。进一步研究发现,在干旱和盐分处理下,VvLBD39 对气孔关闭、ABA 生物合成和 ABA 信号转导有负面影响。此外,在 VvLBD39 转基因烟草中,与活性氧(ROS)清除和胁迫响应相关的基因表达明显下调。综上所述,这些结果表明 VvLBD39 是耐盐和耐旱性的负调控因子,因此是抗旱和抗盐育种的一个很有前景的靶标。
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VvLBD39, a grape LBD transcription factor, regulates plant response to salt and drought stress

Grape (Vitis vinifera L.), as an important deciduous perennial fruit tree, constantly confronts various abiotic stresses such as salinity and drought. The lateral organ boundaries domain (LBD) proteins are a class of plant-specific transcription factors that play pivotal roles in regulating plant growth and responding to abiotic stress. However, the biological function of the LBD transcription factor in grape remains poorly understood. Here, we cloned and characterized the VvLBD39 gene from grape, which contained a highly conserved LBD domain and localized to the cell nucleus. qRT-PCR analyses showed that the expression of VvLBD39 was downregulated upon exposure to NaCl, polyethylene glycol 6000 (PEG6000) and abscisic acid (ABA) treatments, respectively. Overexpression of VvLBD39 in grape calli and Arabidopsis resulted in hypersensitivity to PEG6000 and NaCl stress. Moreover, VvLBD39-overexpressing transgenic tobacco exhibited decreased tolerance to drought and salt stress, as well as insensitivity to exogenous ABA. After drought and salt stress treatments, the chlorophyll content, root length and antioxidant enzyme activity of the transgenic tobacco were lower than those of the wild-type (WT). Conversely, malonic dialdehyde (MDA) content, electronic conductivity, hydrogen peroxide (H2O2) content and superoxide anion (O2-) productivity were markedly elevated in the transgenic tobacco compared to the WT. Further investigations found that VvLBD39 had a negative impact on stomatal closure, ABA biosynthesis and ABA signaling under drought and salt treatments. In addition, the expression of genes related to reactive oxygen species (ROS) scavenging and stress response were significantly downregulated in VvLBD39 transgenic tobacco. Taken together, these results indicated that VvLBD39 functions as a negative regulator of salt and drought tolerance, making it a promising target for drought and salt resistance breeding.

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来源期刊
Environmental and Experimental Botany
Environmental and Experimental Botany 环境科学-环境科学
CiteScore
9.30
自引率
5.30%
发文量
342
审稿时长
26 days
期刊介绍: Environmental and Experimental Botany (EEB) publishes research papers on the physical, chemical, biological, molecular mechanisms and processes involved in the responses of plants to their environment. In addition to research papers, the journal includes review articles. Submission is in agreement with the Editors-in-Chief. The Journal also publishes special issues which are built by invited guest editors and are related to the main themes of EEB. The areas covered by the Journal include: (1) Responses of plants to heavy metals and pollutants (2) Plant/water interactions (salinity, drought, flooding) (3) Responses of plants to radiations ranging from UV-B to infrared (4) Plant/atmosphere relations (ozone, CO2 , temperature) (5) Global change impacts on plant ecophysiology (6) Biotic interactions involving environmental factors.
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