Jun HAN , Zhi-Kai XIAHOU , Ming HAO , Shuai LIN , Ya-Feng SONG
{"title":"基于 \"脾-脑 \"相关性的神仙水提取物改善小鼠睡眠及其对脑组织钙信号通路影响的研究","authors":"Jun HAN , Zhi-Kai XIAHOU , Ming HAO , Shuai LIN , Ya-Feng SONG","doi":"10.1016/j.cjac.2024.100417","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the effects of water extract of the spleen-brain-related mineral drug Shehanshi on mouse sleep.</p></div><div><h3>Methods</h3><p>Shehanshi water extract was subjected to component analysis via X-ray photoelectron spectroscopy. Then, the effects of low-dose (50 mg kg<sup>-1</sup>) and high-dose (100 mg kg<sup>-1</sup>) Shehanshi water extract on mouse sleep were evaluated through behavioral tests such as pentobarbital sodium subthreshold and above-threshold sleep experiments and autonomic activity experiments. Furthermore, transcriptome sequencing and nontarget metabolomics analysis were performed on the spleen and brain tissues of the mice.</p></div><div><h3>Results</h3><p>The Shehanshi water extract contains a total of 30 elements and can reduce sleep latency, increase sleep time, and increase the sleep rate of mice. In the open field experiment, the movement distance of the mice decreased, and the central residence time and rest time increased. Immunoinfiltration analysis and immunohistochemical verification of spleen tissue showed that compared with those in the control group, the immune abundance of neutrophils in the administration groups increased (<em>P</em> < 0.05). Transcriptome data analysis revealed that the Atp1b2 gene was located at the intersection of the spleen and brain and was positively correlated with neutrophil expression but negatively correlated with the expression of the metabolite oleic acid in brain tissue. Immunohistochemical results showed that Atp2a3 protein expression decreased and Plcg1 protein expression increased in the high-dose group, and the difference was statistically significant (<em>P</em> < 0.05). The Atp1b2 protein level in the spleen tissue was positively correlated with that in the brain tissue of the mice (<em>R</em> = 0.829, <em>P</em> = 0.038). Western blotting revealed that Atp1b2 protein levels in the brain and spleen increased significantly in the high-dose group (<em>P</em> < 0.05).</p></div><div><h3>Conclusion</h3><p>The mechanism by which Shehanshi water extract influences sleep may be associated with the expression of genes related to the spleen-brain axis and calcium signaling pathways in brain tissues.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 8","pages":"Article 100417"},"PeriodicalIF":1.2000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000628/pdfft?md5=5ecb9161a16fef867d188513f49e14bc&pid=1-s2.0-S1872204024000628-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Effects of water extract of the spleen-brain-related mineral drug Shehanshi on mouse sleep\",\"authors\":\"Jun HAN , Zhi-Kai XIAHOU , Ming HAO , Shuai LIN , Ya-Feng SONG\",\"doi\":\"10.1016/j.cjac.2024.100417\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>To investigate the effects of water extract of the spleen-brain-related mineral drug Shehanshi on mouse sleep.</p></div><div><h3>Methods</h3><p>Shehanshi water extract was subjected to component analysis via X-ray photoelectron spectroscopy. Then, the effects of low-dose (50 mg kg<sup>-1</sup>) and high-dose (100 mg kg<sup>-1</sup>) Shehanshi water extract on mouse sleep were evaluated through behavioral tests such as pentobarbital sodium subthreshold and above-threshold sleep experiments and autonomic activity experiments. Furthermore, transcriptome sequencing and nontarget metabolomics analysis were performed on the spleen and brain tissues of the mice.</p></div><div><h3>Results</h3><p>The Shehanshi water extract contains a total of 30 elements and can reduce sleep latency, increase sleep time, and increase the sleep rate of mice. In the open field experiment, the movement distance of the mice decreased, and the central residence time and rest time increased. Immunoinfiltration analysis and immunohistochemical verification of spleen tissue showed that compared with those in the control group, the immune abundance of neutrophils in the administration groups increased (<em>P</em> < 0.05). Transcriptome data analysis revealed that the Atp1b2 gene was located at the intersection of the spleen and brain and was positively correlated with neutrophil expression but negatively correlated with the expression of the metabolite oleic acid in brain tissue. Immunohistochemical results showed that Atp2a3 protein expression decreased and Plcg1 protein expression increased in the high-dose group, and the difference was statistically significant (<em>P</em> < 0.05). The Atp1b2 protein level in the spleen tissue was positively correlated with that in the brain tissue of the mice (<em>R</em> = 0.829, <em>P</em> = 0.038). Western blotting revealed that Atp1b2 protein levels in the brain and spleen increased significantly in the high-dose group (<em>P</em> < 0.05).</p></div><div><h3>Conclusion</h3><p>The mechanism by which Shehanshi water extract influences sleep may be associated with the expression of genes related to the spleen-brain axis and calcium signaling pathways in brain tissues.</p></div>\",\"PeriodicalId\":277,\"journal\":{\"name\":\"Chinese Journal of Analytical Chemistry\",\"volume\":\"52 8\",\"pages\":\"Article 100417\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1872204024000628/pdfft?md5=5ecb9161a16fef867d188513f49e14bc&pid=1-s2.0-S1872204024000628-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chinese Journal of Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1872204024000628\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Journal of Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1872204024000628","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Effects of water extract of the spleen-brain-related mineral drug Shehanshi on mouse sleep
Objective
To investigate the effects of water extract of the spleen-brain-related mineral drug Shehanshi on mouse sleep.
Methods
Shehanshi water extract was subjected to component analysis via X-ray photoelectron spectroscopy. Then, the effects of low-dose (50 mg kg-1) and high-dose (100 mg kg-1) Shehanshi water extract on mouse sleep were evaluated through behavioral tests such as pentobarbital sodium subthreshold and above-threshold sleep experiments and autonomic activity experiments. Furthermore, transcriptome sequencing and nontarget metabolomics analysis were performed on the spleen and brain tissues of the mice.
Results
The Shehanshi water extract contains a total of 30 elements and can reduce sleep latency, increase sleep time, and increase the sleep rate of mice. In the open field experiment, the movement distance of the mice decreased, and the central residence time and rest time increased. Immunoinfiltration analysis and immunohistochemical verification of spleen tissue showed that compared with those in the control group, the immune abundance of neutrophils in the administration groups increased (P < 0.05). Transcriptome data analysis revealed that the Atp1b2 gene was located at the intersection of the spleen and brain and was positively correlated with neutrophil expression but negatively correlated with the expression of the metabolite oleic acid in brain tissue. Immunohistochemical results showed that Atp2a3 protein expression decreased and Plcg1 protein expression increased in the high-dose group, and the difference was statistically significant (P < 0.05). The Atp1b2 protein level in the spleen tissue was positively correlated with that in the brain tissue of the mice (R = 0.829, P = 0.038). Western blotting revealed that Atp1b2 protein levels in the brain and spleen increased significantly in the high-dose group (P < 0.05).
Conclusion
The mechanism by which Shehanshi water extract influences sleep may be associated with the expression of genes related to the spleen-brain axis and calcium signaling pathways in brain tissues.
期刊介绍：
Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.
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