He TIAN , Yuyao WEI , Yu HE , Jingwen ZHAO , Yilan OUYANG , Haiying LIU , Lin YI , Zhenqing ZHANG
{"title":"基于光子相关光谱的 PF4/肝素免疫原性复合物的动态粒度研究","authors":"He TIAN , Yuyao WEI , Yu HE , Jingwen ZHAO , Yilan OUYANG , Haiying LIU , Lin YI , Zhenqing ZHANG","doi":"10.1016/j.cjac.2024.100419","DOIUrl":null,"url":null,"abstract":"<div><p>In this work, the process of forming PF4/heparin complex was described based on their sizes measured with PCS. PF4/heparin complexes formed when a small amount of heparin was added to PF4, and they grew to bigger particles when more heparin was added, but they started to decompose after a certain amount of heparin was added. The larger the molecular weight of heparin has, the less amount of heparin is required to form the complex with PF4. This process was verified by the results of the complex surface charge. This method was used to compare the immunogenicity among the different batches of nadroparin after its specificity and stability were validated.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 8","pages":"Article 100419"},"PeriodicalIF":1.2000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000641/pdfft?md5=b33de89adc61dd75e2fba6565d7e882f&pid=1-s2.0-S1872204024000641-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Dynamic particle size study of immunogenic complexes of PF4/heparin based on photon correlation spectroscopy\",\"authors\":\"He TIAN , Yuyao WEI , Yu HE , Jingwen ZHAO , Yilan OUYANG , Haiying LIU , Lin YI , Zhenqing ZHANG\",\"doi\":\"10.1016/j.cjac.2024.100419\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In this work, the process of forming PF4/heparin complex was described based on their sizes measured with PCS. PF4/heparin complexes formed when a small amount of heparin was added to PF4, and they grew to bigger particles when more heparin was added, but they started to decompose after a certain amount of heparin was added. The larger the molecular weight of heparin has, the less amount of heparin is required to form the complex with PF4. This process was verified by the results of the complex surface charge. This method was used to compare the immunogenicity among the different batches of nadroparin after its specificity and stability were validated.</p></div>\",\"PeriodicalId\":277,\"journal\":{\"name\":\"Chinese Journal of Analytical Chemistry\",\"volume\":\"52 8\",\"pages\":\"Article 100419\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1872204024000641/pdfft?md5=b33de89adc61dd75e2fba6565d7e882f&pid=1-s2.0-S1872204024000641-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chinese Journal of Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1872204024000641\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Journal of Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1872204024000641","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Dynamic particle size study of immunogenic complexes of PF4/heparin based on photon correlation spectroscopy
In this work, the process of forming PF4/heparin complex was described based on their sizes measured with PCS. PF4/heparin complexes formed when a small amount of heparin was added to PF4, and they grew to bigger particles when more heparin was added, but they started to decompose after a certain amount of heparin was added. The larger the molecular weight of heparin has, the less amount of heparin is required to form the complex with PF4. This process was verified by the results of the complex surface charge. This method was used to compare the immunogenicity among the different batches of nadroparin after its specificity and stability were validated.
期刊介绍：
Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.
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