{"title":"注射脂多糖和补充缬氨酸的卫生条件对断奶猪生长性能、免疫反应、细菌特征和微生物代谢物的影响","authors":"Bonjin Koo, Chengbo Yang, Charles Martin Nyachoti","doi":"10.1080/1745039X.2024.2382278","DOIUrl":null,"url":null,"abstract":"<p><p>This study investigated the effects of dietary <sub>L</sub>-valine (<b>Val</b>) supplementation and sanitary conditions with lipopolysaccharide injection on growth performance, immune response, and intestinal bacterial profiles and metabolites in weaned pigs. Thirty-two weaned pigs (6.98 ± 0.47 kg) were randomly assigned to treatments in a 2 × 2 factorial arrangement based on dietary Val levels and sanitary conditions (low or high). The pigs were fed either a basal diet containing the standard levels of Val suggested by (NRC), (2012) or a basal diet supplemented with 0.1% <sub>L</sub>-Val. A room designated as a high sanitary room was washed weekly, whereas the designated low sanitary room was not washed throughout the experiment and 5 kg of manure from the nursery pig barn was spread on the pen floors on day 1. All data were analysed using a mixed procedure of SAS, with the individual pen as the experimental unit. The pigs raised in low sanitary conditions exhibited a lower (<i>p</i> < 0.05) average daily gain, average daily feed intake, and gain-to-feed ratio and a higher (<i>p</i> < 0.05) incidence of diarrhoea than those raised in high sanitary conditions during the 14-d experimental period. The pigs in the low sanitary group also had a lower (<i>p</i> < 0.05) concentration of butyrate in the jejunum and a higher (<i>p</i> < 0.05) concentration of NH<sub>3</sub>-N in the colon than those in the high sanitary group. Dietary Val supplementation was reduced (<i>p</i> < 0.05) plasma interleukin (<b>IL</b>)-1β and IL-1 receptor antagonist concentrations as well as isovalerate and NH<sub>3</sub>-N concentrations in the colon, regardless of sanitary conditions. Interactions between dietary Val supplementation and sanitary conditions were observed in the abundances of mRNA-encoding β-defensins 113, 125 and 129 (<i>p</i> < 0.05). In conclusion, dietary Val supplementation beneficially modulates inflammatory responses and microbial metabolites regardless of sanitary conditions while transcriptional levels of β-defensins are regulated by dietary Val supplementation in a manner dependent on housing hygiene conditions.</p>","PeriodicalId":8157,"journal":{"name":"Archives of Animal Nutrition","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of sanitary conditions with lipopolysaccharide injection and dietary valine supplementation on growth performance, immune response, bacterial profile, and microbial metabolites in weaned pigs.\",\"authors\":\"Bonjin Koo, Chengbo Yang, Charles Martin Nyachoti\",\"doi\":\"10.1080/1745039X.2024.2382278\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study investigated the effects of dietary <sub>L</sub>-valine (<b>Val</b>) supplementation and sanitary conditions with lipopolysaccharide injection on growth performance, immune response, and intestinal bacterial profiles and metabolites in weaned pigs. Thirty-two weaned pigs (6.98 ± 0.47 kg) were randomly assigned to treatments in a 2 × 2 factorial arrangement based on dietary Val levels and sanitary conditions (low or high). The pigs were fed either a basal diet containing the standard levels of Val suggested by (NRC), (2012) or a basal diet supplemented with 0.1% <sub>L</sub>-Val. A room designated as a high sanitary room was washed weekly, whereas the designated low sanitary room was not washed throughout the experiment and 5 kg of manure from the nursery pig barn was spread on the pen floors on day 1. All data were analysed using a mixed procedure of SAS, with the individual pen as the experimental unit. The pigs raised in low sanitary conditions exhibited a lower (<i>p</i> < 0.05) average daily gain, average daily feed intake, and gain-to-feed ratio and a higher (<i>p</i> < 0.05) incidence of diarrhoea than those raised in high sanitary conditions during the 14-d experimental period. The pigs in the low sanitary group also had a lower (<i>p</i> < 0.05) concentration of butyrate in the jejunum and a higher (<i>p</i> < 0.05) concentration of NH<sub>3</sub>-N in the colon than those in the high sanitary group. Dietary Val supplementation was reduced (<i>p</i> < 0.05) plasma interleukin (<b>IL</b>)-1β and IL-1 receptor antagonist concentrations as well as isovalerate and NH<sub>3</sub>-N concentrations in the colon, regardless of sanitary conditions. Interactions between dietary Val supplementation and sanitary conditions were observed in the abundances of mRNA-encoding β-defensins 113, 125 and 129 (<i>p</i> < 0.05). In conclusion, dietary Val supplementation beneficially modulates inflammatory responses and microbial metabolites regardless of sanitary conditions while transcriptional levels of β-defensins are regulated by dietary Val supplementation in a manner dependent on housing hygiene conditions.</p>\",\"PeriodicalId\":8157,\"journal\":{\"name\":\"Archives of Animal Nutrition\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of Animal Nutrition\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1080/1745039X.2024.2382278\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Animal Nutrition","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/1745039X.2024.2382278","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
摘要
本研究调查了日粮中补充 L-缬氨酸(Val)和注射脂多糖的卫生条件对断奶猪生长性能、免疫反应、肠道细菌谱和代谢物的影响。将 32 头断奶猪(6.98 ± 0.47 千克)按日粮 Val 水平和卫生条件(低或高)随机分配到 2 × 2 因子排列的处理中。猪被饲喂含有(NRC)(2012 年)建议的标准 Val 水平的基础日粮或添加 0.1% 左旋 Val 的基础日粮。指定为高卫生标准的房间每周清洗一次,而指定为低卫生标准的房间在整个实验期间不清洗,并在第 1 天将 5 公斤来自保育猪舍的粪便撒在猪栏地板上。所有数据均采用 SAS 混合程序进行分析,以单个猪栏为实验单位。在低卫生条件下饲养的猪的结肠中 3-N 含量(p p p p p)低于高卫生条件组。无论卫生条件如何,日粮中补充缬氨酸都会降低结肠中(p IL)-1β 和 IL-1 受体拮抗剂的浓度以及异戊酸和 NH3-N 的浓度。在编码 β-防御素 113、125 和 129 的 mRNA 丰度中,观察到膳食补充 Val 与卫生条件之间的相互作用(p
Effects of sanitary conditions with lipopolysaccharide injection and dietary valine supplementation on growth performance, immune response, bacterial profile, and microbial metabolites in weaned pigs.
This study investigated the effects of dietary L-valine (Val) supplementation and sanitary conditions with lipopolysaccharide injection on growth performance, immune response, and intestinal bacterial profiles and metabolites in weaned pigs. Thirty-two weaned pigs (6.98 ± 0.47 kg) were randomly assigned to treatments in a 2 × 2 factorial arrangement based on dietary Val levels and sanitary conditions (low or high). The pigs were fed either a basal diet containing the standard levels of Val suggested by (NRC), (2012) or a basal diet supplemented with 0.1% L-Val. A room designated as a high sanitary room was washed weekly, whereas the designated low sanitary room was not washed throughout the experiment and 5 kg of manure from the nursery pig barn was spread on the pen floors on day 1. All data were analysed using a mixed procedure of SAS, with the individual pen as the experimental unit. The pigs raised in low sanitary conditions exhibited a lower (p < 0.05) average daily gain, average daily feed intake, and gain-to-feed ratio and a higher (p < 0.05) incidence of diarrhoea than those raised in high sanitary conditions during the 14-d experimental period. The pigs in the low sanitary group also had a lower (p < 0.05) concentration of butyrate in the jejunum and a higher (p < 0.05) concentration of NH3-N in the colon than those in the high sanitary group. Dietary Val supplementation was reduced (p < 0.05) plasma interleukin (IL)-1β and IL-1 receptor antagonist concentrations as well as isovalerate and NH3-N concentrations in the colon, regardless of sanitary conditions. Interactions between dietary Val supplementation and sanitary conditions were observed in the abundances of mRNA-encoding β-defensins 113, 125 and 129 (p < 0.05). In conclusion, dietary Val supplementation beneficially modulates inflammatory responses and microbial metabolites regardless of sanitary conditions while transcriptional levels of β-defensins are regulated by dietary Val supplementation in a manner dependent on housing hygiene conditions.
期刊介绍:
Archives of Animal Nutrition is an international journal covering the biochemical and physiological basis of animal nutrition. Emphasis is laid on original papers on protein and amino acid metabolism, energy transformation, mineral metabolism, vitamin metabolism, nutritional effects on intestinal and body functions in combination with performance criteria, respectively. It furthermore deals with recent developments in practical animal feeding, feedstuff theory, mode of action of feed additives, feedstuff preservation and feedstuff processing. The spectrum covers all relevant animal species including food producing and companion animals, but not aquatic species.
Seldom can priority be given to papers covering more descriptive studies, even if they may be interesting and technically sound or of impact for animal production, or for topics of relevance for only particular regional conditions.