生产系统对 Arouquesa 牛胸长肌蛋白质组学特征的影响。

IF 2.8 2区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of proteomics Pub Date : 2024-07-29 DOI:10.1016/j.jprot.2024.105265
Laura Sacarrão-Birrento , David M. Ribeiro , Antje Dittmann , Susana P. Alves , Laura Kunz , Severiano Silva , Carlos A. Venâncio , André M. de Almeida
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引用次数: 0

摘要

Arouquesa 品种是葡萄牙本土品种,在传统山地系统下生产,需要在不影响牛肉质量的情况下进行改良。这项工作的目的是比较在不同系统下生产的 Arouquesa 动物胸长肌的蛋白质组学特征。60 头断奶仔牛采用了以下饲养模式:传统饲养模式(TF)、传统饲养模式(TF + S1)(9 个月断奶并屠宰)、S1 + S2(5 个月断奶并补充生长剂直至屠宰)以及两个饲养期(TF + S3 和 S3)并补充精饲料。屠宰时,采集胸大肌样本,并使用霰弹枪蛋白质组学工作流程进行分析。结果发现了阿鲁凯萨品种牛肉质量的几个假定标记:VIM、FSCN1、SERPINH1、ALDH1A1、NDUFB5、ANNA1、PDK4、CEMIP2、NDUFB9、PDLIM1、OXCT1、MYH4。这些蛋白质参与肌动蛋白结合、骨骼肌发育和线粒体呼吸链,它们主要影响肉的嫩度和色泽。我们为每组蛋白质鉴定了与影响肉质参数的几个方面有关的不同代谢相关的特定蛋白质。我们的研究结果证明了生产实践与肉质特征之间的联系,这有可能提高认证产品的可追溯性。意义:Arouquesa 品种是在可持续系统中利用自然资源生产的,对葡萄牙北部人口较少的农村地区的经济发展做出了贡献。除经济意义外,生产本土品种还能抵御农村人口减少,维护地方遗产。此外,消费者对产品质量的认识在不断提高,而 PDO 产品有助于满足这一需求。然而,有必要提高产量,以便能够将这些产品销售到产区之外。为了确保可靠的可追溯性,并在产量增加的情况下保持 PDO 标签的特征,产品分析至关重要。因此,蛋白质组学方法可以让人们深入了解生产变化将如何影响牛肉质量,并生成认证生产系统的推定生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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The effect of the production system on the proteomics profiles of the Longissimus thoracis muscle in Arouquesa cattle

Arouquesa cattle breed is an autochthonous Portuguese breed produced under a traditional mountain system that need improvement without affecting beef quality. The aim of this work is to compare the proteomics profiles of the Longissimus thoracis muscle from Arouquesa animals produced under different production systems. Sixty weaners were produced under the following systems: traditional (TF) and traditional with starter feed supplementation (TF + S1) with weaning and slaughtering at 9 months, the S1 + S2 (weaning at 5 months and grower supplement until slaughter) and two rearing periods with finishing supplementation (TF + S3 and S3). Upon slaughter, samples of L. thoracis were taken and analyzed using a shotgun proteomics workflow. Several putative markers of beef quality for the Arouquesa breed were identified: VIM, FSCN1, SERPINH1, ALDH1A1, NDUFB5, ANXA1, PDK4, CEMIP2, NDUFB9, PDLIM1, OXCT1, MYH4. These proteins are involved in actin binding, skeletal muscle development and in the mitochondrial respiratory chain and they can influence mostly meat tenderness and color. We identified specific proteins for each group related to different metabolisms involved in several aspects that affect meat quality parameters. Our results demonstrate the link between production practices and putative meat characteristics, which have the potential to improve the traceability of certified products.

Significance

Arouquesa breed is produced in a sustainable system using natural resources and contributing to the economy of low-populated rural regions in Northern Portugal. Besides their economic relevance, producing autochthonous breeds can counter rural depopulation and maintain local heritage. Additionally, consumer awareness about product quality is increasing and PDO products contribute to satisfying this demand. However, it is necessary to increase production so that it is possible to sell these products outside the production region. To ensure robust traceability and that PDO label characteristics are maintained despite increasing production yield, product analysis is of paramount importance. For this reason, proteomic approaches can provide insight into how production changes will affect beef quality and generate putative biomarkers of certified production systems.

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来源期刊
Journal of proteomics
Journal of proteomics 生物-生化研究方法
CiteScore
7.10
自引率
3.00%
发文量
227
审稿时长
73 days
期刊介绍: Journal of Proteomics is aimed at protein scientists and analytical chemists in the field of proteomics, biomarker discovery, protein analytics, plant proteomics, microbial and animal proteomics, human studies, tissue imaging by mass spectrometry, non-conventional and non-model organism proteomics, and protein bioinformatics. The journal welcomes papers in new and upcoming areas such as metabolomics, genomics, systems biology, toxicogenomics, pharmacoproteomics. Journal of Proteomics unifies both fundamental scientists and clinicians, and includes translational research. Suggestions for reviews, webinars and thematic issues are welcome.
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