白细胞介素-10基因多态性调节埃尔比勒省沙眼衣原体阳性库尔德妇女的不孕风险

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2024-01-01 DOI:10.61186/rbmb.12.4.550
Lawin Ahmed Omar, Suhaila Nafee Darogha
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引用次数: 0

摘要

背景:女性对衣原体感染和生殖道问题的反应存在明显的个体差异。女性白细胞介素-10(IL-10)基因的遗传变异与沙眼衣原体感染反应的差异有关。本研究旨在证明 IL-10 与不孕症的密切关系,并证明 IL-10 (-592 C/A rs1800872) 和 (-1082 A>G rs1800896) 单核苷酸多态性(SNPs)基因在沙眼衣原体感染的易感性和严重程度中的作用:在这项评估研究中,采用酶联免疫吸附试验(ELISA)测定了 134 名不孕症妇女和 50 名健康志愿者的血清 IL-10 浓度。通过四扩增难治性突变系统-PCR(T-ARMS-PCR)分析检测 rs1800872 和 rs1800896 SNPs 基因的基因分型:结果:两组女性患者的抗衣原体IgM抗体均呈阳性,但不同病例的反应强度不同。同时,通过 PCR 检测,不孕妇女生殖器沙眼衣原体感染率为 46.2%。除子宫内膜异位症(Endo)不孕症外,不孕妇女血清中的IL10浓度均低于健康参与者,而沙眼衣原体阳性不孕妇女血清中的IL10浓度高于沙眼衣原体阴性不孕妇女。在 rs1800872 中,CA 基因型和 C 等位基因与不孕风险的增加有关,但多囊卵巢综合征(PCOS)除外,其等位基因为 A。就 rs1800896 而言,AG 基因型和 G 等位基因显示出更大的不孕风险:我们的研究结果证实,rs1800872 和 rs1800896 基因多态性与沙眼衣原体感染风险增加有关。
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Interleukin-10 Gene Polymorphisms Modulate the Risk of Infertility in Chlamydia trachomatis Positive Kurdish Women in Erbil Province.

Background: There is evident inter-individual variability in women's responses to Chlamydial infections and reproductive tract problems. Women's genetic variations within the Interleukin-10 (IL-10) gene have been linked to variances in response to Chlamydia trachomatis infection. This study was aimed to demonstrate the profound association of IL-10 with infertility and demonstrate the role of IL-10 (-592 C/A rs1800872) and (-1082 A>G rs1800896) single nucleotide polymorphism (SNPs) gene in the susceptibility and severity of a C. trachomatis infection.

Methods: In this evaluation study, serum IL-10 concentration was measured in 134 women diagnosed with infertility and 50 healthy volunteers by enzyme-linked immunosorbent assay (ELISA). The tetra-amplification refractory mutation system-PCR (T-ARMS-PCR) analysis was performed to detect the genotyping of the rs1800872 and rs1800896 SNPs genes.

Results: Both female groups were positive for anti-chlamydial IgM antibody, but the intensity of response differed between cases. At the same time, the incidence of genital C. trachomatis by PCR was 46.2% in infertile women. The serum concentration of IL10 was lower in infertile women than healthy participants and higher in infertile C. trachomatis -positive women compared to infertile C. trachomatis-negative in all groups except endometriosis (Endo) infertility. In rs1800872, the CA genotype and C allele are associated with an increased risk for infertility, except in polycystic ovarian syndrome (PCOS), which is an A allele. In the case of rs1800896, the AG genotype and G allele show a greater risk for infertility.

Conclusions: Our results confirmed that rs1800872 and rs1800896 gene polymorphisms were associated with an increased risk of C. trachomatis infection.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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