两种氟释放胶带对变异链球菌生物膜的抗菌作用

IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Clinical Pediatric Dentistry Pub Date : 2024-07-01 Epub Date: 2024-07-03 DOI:10.22514/jocpd.2024.086
Ok Hyung Nam, Tae-Young Park, Seo-Rin Jeong, Jonghyun Shin, Myeong-Kwan Jih
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引用次数: 0

摘要

释放氟化物的胶带已被开发为一种新型氟化物输送剂。然而,作为防龋剂的应用仍未得到充分探索。本研究旨在评估两种释放氟化物的胶带对突变杆状病毒生物膜的抗菌活性。研究了两种聚乙烯醇(PVA)胶带:(i) 含氟-PVA(F-PVA)胶带,(ii) 含有拉普兰的 F-PVA(PF-PVA)胶带。用这些胶带培养和处理 S. mutan 菌株。使用琼脂扩散试验、场发射扫描电子显微镜(FE-SEM)和激光共聚焦扫描显微镜(CLSM)对抗菌效果进行了评估。在 48 小时和 72 小时内,F-PVA 胶带的抑菌区直径高于 PF-PVA,但 F-PVA 和 PF-PVA 的抑菌效果无显著差异(p > 0.05)。与 PF-PVA 相比,F-PVA 胶带中突变菌的生物体积和细胞外聚合物质明显减少(p < 0.05)。FE-SEM 显微照片显示,F-PVA 中的变异杆菌定植较少。与 PF-PVA 相比,F-PVA 具有更好的抗突变菌活性。
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Antimicrobial effect of two fluoride-releasing adhesive tapes on Streptococcus mutans biofilm.

Fluoride-releasing adhesive tapes have been developed as a new fluoride delivery agent. However, application as caries prevention agents remains underexplored. This study aimed at evaluating the antimicrobial activity of two fluoride-releasing adhesive tapes against S. mutans biofilm. Two polyvinyl alcohol (PVA) tapes were investigated: (i) a fluoride-PVA (F-PVA) tape, (ii) a pullulan incorporated F-PVA (PF-PVA) tape. S. mutan strains were cultured and treated with the tapes. Antimicrobial effects were evaluated using the agar diffusion test, field-emission scanning electron microscopy (FE-SEM), and confocal laser scanning microscopy (CLSM). F-PVA tapes showed higher inhibition-zone diameters than PF-PVA at 48 h and 72 h. However, there were no significant differences (p > 0.05) between the effects of F-PVA and PF-PVA. The bio-volume of S. mutans and extracellular polymeric substances significantly decreased in the F-PVA tapes than in the PF-PVA tapes (p < 0.05). FE-SEM micrographs revealed less S. mutans colonization in F-PVA. F-PVA exhibited better antimicrobial activity against S. mutans than PF-PVA.

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来源期刊
Journal of Clinical Pediatric Dentistry
Journal of Clinical Pediatric Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-PEDIATRICS
CiteScore
1.80
自引率
7.70%
发文量
47
期刊介绍: The purpose of The Journal of Clinical Pediatric Dentistry is to provide clinically relevant information to enable the practicing dentist to have access to the state of the art in pediatric dentistry. From prevention, to information, to the management of different problems encountered in children''s related medical and dental problems, this peer-reviewed journal keeps you abreast of the latest news and developments related to pediatric dentistry.
期刊最新文献
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