四个核型不同的 Papaver 物种的中心粒景观为染色体进化和物种分化提供了启示。

IF 11.1 Q1 CELL BIOLOGY Cell genomics Pub Date : 2024-08-14 Epub Date: 2024-07-30 DOI:10.1016/j.xgen.2024.100626
Shenghan Gao, Yanyan Jia, Hongtao Guo, Tun Xu, Bo Wang, Stephen J Bush, Shijie Wan, Yimeng Zhang, Xiaofei Yang, Kai Ye
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引用次数: 0

摘要

由于中心粒由大量串联重复的卫星 DNA 阵列组成,阻碍了高质量的组装,因此了解中心粒在染色体进化和物种分化中的作用变得更加复杂。在这里,我们利用长线程测序技术为四个核型不同的巴布亚草物种(P. setigerum (2n = 44)、P. somniferum (2n = 22)、P. rhoeas (2n = 14) 和 P. bracteatum (2n = 14))生成了近乎完整的基因组组装,总共代表了 45 个无间隙中心粒。我们发现了四个中心粒卫星(cenSat)家族,并通过实验验证了其中两个代表。对于两个全多倍体基因组(P. somniferum 和 P. setigerum),我们描述了每个卫星的亚基因组分布,并确定了杂交后中心粒进化的 "同质化 "阶段。中心粒周围区域的种间比较进一步揭示了广泛的由中心粒介导的染色体重排。综合这些结果,我们提出了一个研究杂交后中心粒竞争的模型,并进一步揭示了中心粒在物种演化中的复杂作用。
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The centromere landscapes of four karyotypically diverse Papaver species provide insights into chromosome evolution and speciation.

Understanding the roles played by centromeres in chromosome evolution and speciation is complicated by the fact that centromeres comprise large arrays of tandemly repeated satellite DNA, which hinders high-quality assembly. Here, we used long-read sequencing to generate nearly complete genome assemblies for four karyotypically diverse Papaver species, P. setigerum (2n = 44), P. somniferum (2n = 22), P. rhoeas (2n = 14), and P. bracteatum (2n = 14), collectively representing 45 gapless centromeres. We identified four centromere satellite (cenSat) families and experimentally validated two representatives. For the two allopolyploid genomes (P. somniferum and P. setigerum), we characterized the subgenomic distribution of each satellite and identified a "homogenizing" phase of centromere evolution in the aftermath of hybridization. An interspecies comparison of the peri-centromeric regions further revealed extensive centromere-mediated chromosome rearrangements. Taking these results together, we propose a model for studying cenSat competition after hybridization and shed further light on the complex role of the centromere in speciation.

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