Anna Herrmann, Srinivas Abbina, Nuthan Vikas Bathula, Peyman Malek Mohammadi Nouri, Irina Chafeeva, Iren Constantinescu, Emaan Abbasi, Usama Abbasi, Matthew Drayton, Haiming Daniel Luo, Haisle Moon, Arshdeep Gill, Yu Xi, Allan K. Bertram, Caigan Du, Rainer Haag, Anna K. Blakney, Jayachandran N. Kizhakkedathu
{"title":"一种可保护蛋白质治疗剂和 RNA 脂质纳米颗粒免受冷冻、加热和冻干应力影响的超水合聚合物","authors":"Anna Herrmann, Srinivas Abbina, Nuthan Vikas Bathula, Peyman Malek Mohammadi Nouri, Irina Chafeeva, Iren Constantinescu, Emaan Abbasi, Usama Abbasi, Matthew Drayton, Haiming Daniel Luo, Haisle Moon, Arshdeep Gill, Yu Xi, Allan K. Bertram, Caigan Du, Rainer Haag, Anna K. Blakney, Jayachandran N. Kizhakkedathu","doi":"10.1002/adfm.202406878","DOIUrl":null,"url":null,"abstract":"<p>RNA and protein-based therapeutics constitute almost half of recent drug approvals and receive considerable attention within biotechnology industries. Ensuring their stability and longevity in the context of heat, freezing, and lyophilization processes are paramount to a successful deployment. However, the advancement of formulations designed to achieve this goal is still in its nascent phase. To address these challenges, a new class of semi-dendritic hydrophilic polymer with extended linear units is reported, which showcase very high hydration. These novel polymers demonstrated exceptional efficacy in preserving messenger RNA- (mRNA-) and self-amplifying RNA- (saRNA-) lipid nanoparticles during freezing and lyophilization. Additionally, they have been found to protect therapeutic proteins against external stressors such as freezing, heat, and lyophilization. These polymers are non-toxic, which enables their utilization at high concentrations and eliminates the requirement for removal prior to administration. It is found that their unique topology contributes to the high hydration. These excipients are anticipated to create new prospects in biotechnology, food science, and cryopreservation.</p>","PeriodicalId":112,"journal":{"name":"Advanced Functional Materials","volume":null,"pages":null},"PeriodicalIF":18.5000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adfm.202406878","citationCount":"0","resultStr":"{\"title\":\"An Ultrahydrating Polymer that Protects Protein Therapeutics and RNA-Lipid Nanoparticles Against Freezing, Heat and Lyophilization Stress\",\"authors\":\"Anna Herrmann, Srinivas Abbina, Nuthan Vikas Bathula, Peyman Malek Mohammadi Nouri, Irina Chafeeva, Iren Constantinescu, Emaan Abbasi, Usama Abbasi, Matthew Drayton, Haiming Daniel Luo, Haisle Moon, Arshdeep Gill, Yu Xi, Allan K. Bertram, Caigan Du, Rainer Haag, Anna K. Blakney, Jayachandran N. 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These polymers are non-toxic, which enables their utilization at high concentrations and eliminates the requirement for removal prior to administration. It is found that their unique topology contributes to the high hydration. 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An Ultrahydrating Polymer that Protects Protein Therapeutics and RNA-Lipid Nanoparticles Against Freezing, Heat and Lyophilization Stress
RNA and protein-based therapeutics constitute almost half of recent drug approvals and receive considerable attention within biotechnology industries. Ensuring their stability and longevity in the context of heat, freezing, and lyophilization processes are paramount to a successful deployment. However, the advancement of formulations designed to achieve this goal is still in its nascent phase. To address these challenges, a new class of semi-dendritic hydrophilic polymer with extended linear units is reported, which showcase very high hydration. These novel polymers demonstrated exceptional efficacy in preserving messenger RNA- (mRNA-) and self-amplifying RNA- (saRNA-) lipid nanoparticles during freezing and lyophilization. Additionally, they have been found to protect therapeutic proteins against external stressors such as freezing, heat, and lyophilization. These polymers are non-toxic, which enables their utilization at high concentrations and eliminates the requirement for removal prior to administration. It is found that their unique topology contributes to the high hydration. These excipients are anticipated to create new prospects in biotechnology, food science, and cryopreservation.
期刊介绍:
Firmly established as a top-tier materials science journal, Advanced Functional Materials reports breakthrough research in all aspects of materials science, including nanotechnology, chemistry, physics, and biology every week.
Advanced Functional Materials is known for its rapid and fair peer review, quality content, and high impact, making it the first choice of the international materials science community.