细菌防御系统 MADS 与 CRISPR-Cas 相互作用,限制噬菌体感染和逃逸

IF 20.6 1区 医学 Q1 MICROBIOLOGY Cell host & microbe Pub Date : 2024-08-01 DOI:10.1016/j.chom.2024.07.005
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引用次数: 0

摘要

细菌与寄生虫之间的持续军备竞赛导致细菌防御系统的多样性,许多细菌携带多种防御系统。在这里,我们报告发现了一种在系统发育上广泛存在的防御系统,被称为甲基化相关防御系统(MADS),它分布于革兰氏阳性和革兰氏阴性细菌中。MADS 与原生宿主的 CRISPR-Cas 系统相互作用,对噬菌体产生强大而持久的抵抗力。虽然噬菌体可以获得由表观遗传介导的对 MADS 的抗性,但 MADS 和 CRISPR-Cas 系统的共存限制了逃逸的出现。MADS 由 8 个基因组成,具有预测的核酸酶、ATP 酶、激酶和甲基转移酶结构域,其中大多数基因对于自我/非自我分辨、DNA 限制或两者都至关重要。与其他原核生物防御系统相比,MADS 和类似 MADS 系统的复杂基因结构表明,它们具有高度复杂的感知感染、激活防御和/或干扰的机制。
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The bacterial defense system MADS interacts with CRISPR-Cas to limit phage infection and escape

The constant arms race between bacteria and their parasites has resulted in a large diversity of bacterial defenses, with many bacteria carrying multiple systems. Here, we report the discovery of a phylogenetically widespread defense system, coined methylation-associated defense system (MADS), which is distributed across gram-positive and gram-negative bacteria. MADS interacts with a CRISPR-Cas system in its native host to provide robust and durable resistance against phages. While phages can acquire epigenetic-mediated resistance against MADS, co-existence of MADS and a CRISPR-Cas system limits escape emergence. MADS comprises eight genes with predicted nuclease, ATPase, kinase, and methyltransferase domains, most of which are essential for either self/non-self discrimination, DNA restriction, or both. The complex genetic architecture of MADS and MADS-like systems, relative to other prokaryotic defenses, points toward highly elaborate mechanisms of sensing infections, defense activation, and/or interference.

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来源期刊
Cell host & microbe
Cell host & microbe 生物-微生物学
CiteScore
45.10
自引率
1.70%
发文量
201
审稿时长
4-8 weeks
期刊介绍: Cell Host & Microbe is a scientific journal that was launched in March 2007. The journal aims to provide a platform for scientists to exchange ideas and concepts related to the study of microbes and their interaction with host organisms at a molecular, cellular, and immune level. It publishes novel findings on a wide range of microorganisms including bacteria, fungi, parasites, and viruses. The journal focuses on the interface between the microbe and its host, whether the host is a vertebrate, invertebrate, or plant, and whether the microbe is pathogenic, non-pathogenic, or commensal. The integrated study of microbes and their interactions with each other, their host, and the cellular environment they inhabit is a unifying theme of the journal. The published work in Cell Host & Microbe is expected to be of exceptional significance within its field and also of interest to researchers in other areas. In addition to primary research articles, the journal features expert analysis, commentary, and reviews on current topics of interest in the field.
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