Ganapaty Manickavasagam, Mardiana Saaid, Muhammad Hakimin Shafie, Vuanghao Lim, Ainolsyakira Mohd Rodhi
{"title":"不同产地马来西亚无刺蜂蜂蜜中单糖、糖酸、稳定碳同位素和挥发性有机化合物的化学计量学研究","authors":"Ganapaty Manickavasagam, Mardiana Saaid, Muhammad Hakimin Shafie, Vuanghao Lim, Ainolsyakira Mohd Rodhi","doi":"10.1007/s13738-024-03074-y","DOIUrl":null,"url":null,"abstract":"<div><p>This research aimed to establish the quantification profiling of monosaccharides, sugar acids, stable carbon isotopes, and volatile organic compounds in stingless bee honey produced by <i>Heterotrigona itama</i> (<i>H. itama</i>) bee species employed with geographical discrimination study using chemometrics. In the present study, glucose (730.46–1948.99 µM) was detected in all honey samples; however, mannose (113.78 µM) and fucose (26.38 µM) were detected in only one sample (B2). On the other hand, glucuronic acid, galacturonic acid, ribose, rhamnose, galactose, xylose, and arabinose were absent in all the samples. <i>δ</i><sup>13</sup>C values demonstrated that nectar composition in samples of this study originated from C-3 plants and no possible adulteration was deduced. Besides, a wide range of fifty-three volatile organic compounds (VOCs) was detected across the samples. The partial least square–discriminant analysis (PLS-DA) model illustrated an excellent geographical discrimination pattern of samples based on the studied variables. The PLS-DA model was validated using 10-fold cross-validation (R<sup>2</sup> = 0.84, Q<sup>2 </sup>= 0.95, and accuracy = 0.93) and permutation test (<i>p</i> = 0.008). Variable importance of projection (VIP) exemplified thirteen VOCs (VIP > 1.4, <i>p</i> < 0.01) significant in the discrimination pattern and can be used as potent chemical markers. However, monosaccharides, sugar acids, and carbon isotopes were insignificant (VIP < 1.4,<i> p</i> < 0.01) in discriminating the stingless bee honey according to their geographical origins.</p></div>","PeriodicalId":676,"journal":{"name":"Journal of the Iranian Chemical Society","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chemometrics exploration of monosaccharides, sugar acids, stable carbon isotopes, and volatile organic compounds in Malaysian stingless bee honey from different geographical origins\",\"authors\":\"Ganapaty Manickavasagam, Mardiana Saaid, Muhammad Hakimin Shafie, Vuanghao Lim, Ainolsyakira Mohd Rodhi\",\"doi\":\"10.1007/s13738-024-03074-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This research aimed to establish the quantification profiling of monosaccharides, sugar acids, stable carbon isotopes, and volatile organic compounds in stingless bee honey produced by <i>Heterotrigona itama</i> (<i>H. itama</i>) bee species employed with geographical discrimination study using chemometrics. In the present study, glucose (730.46–1948.99 µM) was detected in all honey samples; however, mannose (113.78 µM) and fucose (26.38 µM) were detected in only one sample (B2). On the other hand, glucuronic acid, galacturonic acid, ribose, rhamnose, galactose, xylose, and arabinose were absent in all the samples. <i>δ</i><sup>13</sup>C values demonstrated that nectar composition in samples of this study originated from C-3 plants and no possible adulteration was deduced. Besides, a wide range of fifty-three volatile organic compounds (VOCs) was detected across the samples. The partial least square–discriminant analysis (PLS-DA) model illustrated an excellent geographical discrimination pattern of samples based on the studied variables. The PLS-DA model was validated using 10-fold cross-validation (R<sup>2</sup> = 0.84, Q<sup>2 </sup>= 0.95, and accuracy = 0.93) and permutation test (<i>p</i> = 0.008). Variable importance of projection (VIP) exemplified thirteen VOCs (VIP > 1.4, <i>p</i> < 0.01) significant in the discrimination pattern and can be used as potent chemical markers. However, monosaccharides, sugar acids, and carbon isotopes were insignificant (VIP < 1.4,<i> p</i> < 0.01) in discriminating the stingless bee honey according to their geographical origins.</p></div>\",\"PeriodicalId\":676,\"journal\":{\"name\":\"Journal of the Iranian Chemical Society\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Iranian Chemical Society\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13738-024-03074-y\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Iranian Chemical Society","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s13738-024-03074-y","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Chemometrics exploration of monosaccharides, sugar acids, stable carbon isotopes, and volatile organic compounds in Malaysian stingless bee honey from different geographical origins
This research aimed to establish the quantification profiling of monosaccharides, sugar acids, stable carbon isotopes, and volatile organic compounds in stingless bee honey produced by Heterotrigona itama (H. itama) bee species employed with geographical discrimination study using chemometrics. In the present study, glucose (730.46–1948.99 µM) was detected in all honey samples; however, mannose (113.78 µM) and fucose (26.38 µM) were detected in only one sample (B2). On the other hand, glucuronic acid, galacturonic acid, ribose, rhamnose, galactose, xylose, and arabinose were absent in all the samples. δ13C values demonstrated that nectar composition in samples of this study originated from C-3 plants and no possible adulteration was deduced. Besides, a wide range of fifty-three volatile organic compounds (VOCs) was detected across the samples. The partial least square–discriminant analysis (PLS-DA) model illustrated an excellent geographical discrimination pattern of samples based on the studied variables. The PLS-DA model was validated using 10-fold cross-validation (R2 = 0.84, Q2 = 0.95, and accuracy = 0.93) and permutation test (p = 0.008). Variable importance of projection (VIP) exemplified thirteen VOCs (VIP > 1.4, p < 0.01) significant in the discrimination pattern and can be used as potent chemical markers. However, monosaccharides, sugar acids, and carbon isotopes were insignificant (VIP < 1.4, p < 0.01) in discriminating the stingless bee honey according to their geographical origins.
期刊介绍:
JICS is an international journal covering general fields of chemistry. JICS welcomes high quality original papers in English dealing with experimental, theoretical and applied research related to all branches of chemistry. These include the fields of analytical, inorganic, organic and physical chemistry as well as the chemical biology area. Review articles discussing specific areas of chemistry of current chemical or biological importance are also published. JICS ensures visibility of your research results to a worldwide audience in science. You are kindly invited to submit your manuscript to the Editor-in-Chief or Regional Editor. All contributions in the form of original papers or short communications will be peer reviewed and published free of charge after acceptance.