肾上腺素能激动剂可激活小鼠上丘脑核永生化神经元细胞的转录活性

IF 8.3 1区 医学 Q1 ENDOCRINOLOGY & METABOLISM Journal of Pineal Research Pub Date : 2024-08-02 DOI:10.1111/jpi.12999
Monica Langiu, Faramarz Dehghani, Urszula Hohmann, Philipp Bechstein, Oliver Rawashdeh, Abdelhaq Rami, Erik Maronde
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引用次数: 0

摘要

下丘脑上核(SCN)是哺乳动物的中枢昼夜节律振荡器。SCN 产生的主要神经递质是γ-氨基丁酸、精氨酸-加压素(AVP)、血管活性肠肽(VIP)、垂体衍生的腺苷酸环化酶激活肽(PACAP)、促动素 2、神经生长因子 S 和胃泌素释放肽(GRP)。除此之外,在 SCN 中还检测到儿茶酚胺及其受体。在本研究中,我们通过免疫组化、免疫细胞化学和药理学技术证实了在 SCN 和小鼠 SCN 衍生的永生细胞系中存在 β 肾上腺素能受体。然后,我们研究了β肾上腺素能激动剂和拮抗剂对cAMP调节因子(CRE)信号传导的影响。此外,我们还研究了β肾上腺素能信号传导与影响平行信号传导途径的物质之间的相互作用。我们的研究结果对压力(肾上腺素升高)对生物钟的作用具有潜在的影响,并可以解释作为抗高血压药物的β受体阻滞剂的一些副作用。
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Adrenergic Agonists Activate Transcriptional Activity in Immortalized Neuronal Cells From the Mouse Suprachiasmatic Nucleus

The suprachiasmatic nucleus of the hypothalamus (SCN) houses the central circadian oscillator of mammals. The main neurotransmitters produced in the SCN are γ-amino-butyric acid, arginine-vasopressin (AVP), vasoactive intestinal peptide (VIP), pituitary-derived adenylate cyclase-activating peptide (PACAP), prokineticin 2, neuromedin S, and gastrin-releasing peptide (GRP). Apart from these, catecholamines and their receptors were detected in the SCN as well. In this study, we confirmed the presence of β-adrenergic receptors in SCN and a mouse SCN-derived immortalized cell line by immunohistochemical, immuno-cytochemical, and pharmacological techniques. We then characterized the effects of β-adrenergic agonists and antagonists on cAMP-regulated element (CRE) signaling. Moreover, we investigated the interaction of β-adrenergic signaling with substances influencing parallel signaling pathways. Our findings have potential implications on the role of stress (elevated adrenaline) on the biological clock and may explain some of the side effects of β-blockers applied as anti-hypertensive drugs.

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来源期刊
Journal of Pineal Research
Journal of Pineal Research 医学-内分泌学与代谢
CiteScore
17.70
自引率
4.90%
发文量
66
审稿时长
1 months
期刊介绍: The Journal of Pineal Research welcomes original scientific research on the pineal gland and melatonin in vertebrates, as well as the biological functions of melatonin in non-vertebrates, plants, and microorganisms. Criteria for publication include scientific importance, novelty, timeliness, and clarity of presentation. The journal considers experimental data that challenge current thinking and welcomes case reports contributing to understanding the pineal gland and melatonin research. Its aim is to serve researchers in all disciplines related to the pineal gland and melatonin.
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