从Syzygium cumini (L.) Skeels种子提取物中绿色合成银纳米粒子及其潜在的药物应用。

Q2 Pharmacology, Toxicology and Pharmaceutics Pharmaceutical nanotechnology Pub Date : 2024-07-31 DOI:10.2174/0122117385316957240710045706

Apsara Kavital, Murigendra B Hiremath, Ritesh Vernekar, Pradeep S Goudar

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引用次数: 0

摘要

目的：纳米技术被认为是生物医学领域发展最快的领域之一。因此，本研究采用绿色方法从茜草种子提取物中合成银纳米粒子：通过紫外可见光谱、傅立叶变换红外光谱、场发射扫描电子显微镜、原子力显微镜、X射线衍射和能量色散 X 射线对合成的纳米粒子进行表征。它们的抗氧化和抗炎活性通过 DPPH（2,2-二苯基-1-吡啶肼）、PM（磷钼）测定和白蛋白变性测定进行了评估。此外，还使用琼脂井扩散法研究了纳米颗粒对革兰氏阳性菌和革兰氏阴性菌的抗菌活性。此外，还通过α-淀粉酶和α-葡萄糖苷酶抑制实验研究了纳米颗粒的抗糖尿病活性：结果：430 纳米波长处的表面等离子共振证实了银纳米粒子的形成。银纳米粒子稳定，呈球形，大小在 30 至 90 nm 之间。银纳米粒子的 DPPH 抑制率从 7.91±0.39% 到 68.35±0.76% 不等。对白蛋白变性的抑制率与双氯芬酸相当。此外，抗菌活性结果表明，所有测试细菌的抑菌区在 14.33±0.58 至 25.33±0.58 mm 之间，其中蜡样芽孢杆菌更易受影响。此外，α-淀粉酶和α-葡萄糖苷酶的抑制率分别为 19.91±0.15% 至 61.43±0.31% 和 15.26±0.11% 至 55.38±0.20%：本研究首次尝试利用小茴香种子提取物合成的银纳米粒子进行抗糖尿病活性研究。该研究表明，这些纳米颗粒可作为一种高效的抗氧化、抗炎、抗菌和抗糖尿病药物在制药业中得到很好的应用。

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Green Synthesis of Silver Nanoparticles from Syzygium cumini (L.) Skeels Seed Extract and their Potential Medicinal Applications.

Aim: Nanotechnology is considered as one of the fastest-developing areas in the biomedicine field. Hence, the green synthesis of silver nanoparticles from Syzygium cumini seed extract was carried out in this study.

Methodology: The synthesized nanoparticles were characterized by UV-Vis spectroscopy, FTIR (Fourier transform infrared), FE-SEM (Field Emission scanning electron microscopic), AFM (Atomic Force Microscope), XRD (X-ray diffraction), and EDX (Energy dispersive X-Ray). Their antioxidant and anti-inflammatory activity were evaluated by DPPH (2,2-diphenyl-1- picrylhydrazyl), PM (Phosphomolybdenum) assay, and albumin denaturation assay. Further, the antibacterial activity of the nanoparticles was studied against Gram-positive and Gram-negative bacteria using the agar well diffusion method. In addition, the antidiabetic activity of nanoparticles was studied by α-amylase and α-glucosidase inhibition assays.

Results: The surface plasmon resonance at 430 nm confirmed the formation of silver nanoparticles. They were stable and spherical in shape, with sizes ranging from 30 to 90 nm. The DPPH inhibition % of silver nanoparticles varied from 7.91±0.39% to 68.35±0.76%. The % inhibition of albumin denaturation was comparable to the diclofenac. Further, the results of antibacterial activity revealed that the zone of inhibition for all the test bacteria varied from 14.33±0.58 to 25.33±0.58 mm, where B. cereus was more susceptible. In addition, the % inhibition of α-amylase and α-glucosidase varied from 19.91±0.15% to 61.43±0.31% and 15.26±0.11% to 55.38±0.20%, respectively.

Conclusion: This study is the first attempt of utilizing the silver nanoparticles synthesized from S. cumini seed extract for antidiabetic activity. The study suggests that these nanoparticles could be well utilized in pharmaceutical industries as an efficient antioxidant, anti-inflammatory, antibacterial, and antidiabetic drug.

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来源期刊

Pharmaceutical nanotechnology Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

4.20

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0.00%

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期刊介绍： Pharmaceutical Nanotechnology publishes original manuscripts, full-length/mini reviews, thematic issues, rapid technical notes and commentaries that provide insights into the synthesis, characterisation and pharmaceutical (or diagnostic) application of materials at the nanoscale. The nanoscale is defined as a size range of below 1 µm. Scientific findings related to micro and macro systems with functionality residing within features defined at the nanoscale are also within the scope of the journal. Manuscripts detailing the synthesis, exhaustive characterisation, biological evaluation, clinical testing and/ or toxicological assessment of nanomaterials are of particular interest to the journal’s readership. Articles should be self contained, centred around a well founded hypothesis and should aim to showcase the pharmaceutical/ diagnostic implications of the nanotechnology approach. Manuscripts should aim, wherever possible, to demonstrate the in vivo impact of any nanotechnological intervention. As reducing a material to the nanoscale is capable of fundamentally altering the material’s properties, the journal’s readership is particularly interested in new characterisation techniques and the advanced properties that originate from this size reduction. Both bottom up and top down approaches to the realisation of nanomaterials lie within the scope of the journal.