{"title":"生物测定指导下从白术中分离抗菌和消炎成分。","authors":"","doi":"10.1016/j.phytochem.2024.114232","DOIUrl":null,"url":null,"abstract":"<div><p>A bioassay-guided isolation from <em>Atractylodes lancea</em> (Thunb.) DC. obtained 22 compounds, including eight previously undescribed sesquiterpenoids and polyacetylenes (<strong>1</strong>, <strong>3</strong> and <strong>12</strong>–<strong>17</strong>), as well as fourteen known analogues, and their structures were confirmed by extensive spectroscopic methods. This study evaluated their antibacterial activity against methicillin resistant <em>Staphylococcus aureus</em> (MRSA) for the first time, as well as anti-inflammatory activity. Most of them, including new compounds, showed varying degrees of antibacterial activity against <em>S. aureus</em> and MRSA. Notably, compound <strong>21</strong> exhibited significant antibacterial activity against four different bacteria (MIC 6.25–20.00 μg/mL). This suggested that <strong>21</strong> may have the potential to be developed into a broad-spectrum antibacterial agent. Moreover, except for <strong>9</strong> and <strong>11</strong>, most compounds exhibited great anti-inflammatory activity (IC<sub>50</sub> 1.92–37.91 μM), and iNOS might be a potential target of these compounds according to the molecular docking analysis.</p></div>","PeriodicalId":20170,"journal":{"name":"Phytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bioassay-guided isolation of antibacterial and anti-inflammatory components from Atractylodes lancea\",\"authors\":\"\",\"doi\":\"10.1016/j.phytochem.2024.114232\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A bioassay-guided isolation from <em>Atractylodes lancea</em> (Thunb.) DC. obtained 22 compounds, including eight previously undescribed sesquiterpenoids and polyacetylenes (<strong>1</strong>, <strong>3</strong> and <strong>12</strong>–<strong>17</strong>), as well as fourteen known analogues, and their structures were confirmed by extensive spectroscopic methods. This study evaluated their antibacterial activity against methicillin resistant <em>Staphylococcus aureus</em> (MRSA) for the first time, as well as anti-inflammatory activity. Most of them, including new compounds, showed varying degrees of antibacterial activity against <em>S. aureus</em> and MRSA. Notably, compound <strong>21</strong> exhibited significant antibacterial activity against four different bacteria (MIC 6.25–20.00 μg/mL). This suggested that <strong>21</strong> may have the potential to be developed into a broad-spectrum antibacterial agent. Moreover, except for <strong>9</strong> and <strong>11</strong>, most compounds exhibited great anti-inflammatory activity (IC<sub>50</sub> 1.92–37.91 μM), and iNOS might be a potential target of these compounds according to the molecular docking analysis.</p></div>\",\"PeriodicalId\":20170,\"journal\":{\"name\":\"Phytochemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2024-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0031942224002693\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0031942224002693","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Bioassay-guided isolation of antibacterial and anti-inflammatory components from Atractylodes lancea
A bioassay-guided isolation from Atractylodes lancea (Thunb.) DC. obtained 22 compounds, including eight previously undescribed sesquiterpenoids and polyacetylenes (1, 3 and 12–17), as well as fourteen known analogues, and their structures were confirmed by extensive spectroscopic methods. This study evaluated their antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA) for the first time, as well as anti-inflammatory activity. Most of them, including new compounds, showed varying degrees of antibacterial activity against S. aureus and MRSA. Notably, compound 21 exhibited significant antibacterial activity against four different bacteria (MIC 6.25–20.00 μg/mL). This suggested that 21 may have the potential to be developed into a broad-spectrum antibacterial agent. Moreover, except for 9 and 11, most compounds exhibited great anti-inflammatory activity (IC50 1.92–37.91 μM), and iNOS might be a potential target of these compounds according to the molecular docking analysis.
期刊介绍:
Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.