Gabriel DeNiro, Kathryn Que, Trevor Fujimoto, Soo Min Koo, Bridget Schneider, Anandaroop Mukhopadhyay, Jeong Kim, Anandi Sawant, Tuan Andrew Nguyen
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引用次数: 0
摘要
该面板旨在描述小鼠肿瘤微环境和小鼠淋巴组织（如脾脏）中的免疫细胞状况。例如，利用 MC-38 小鼠合成肿瘤模型，我们证明可以测量 CD4 T 细胞、CD8 T 细胞、调节性 T 细胞、NK 细胞、B 细胞、巨噬细胞、粒细胞、单核细胞和树突状细胞的频率和功能状态。该面板特别适用于了解免疫细胞浸润极低的 "冷 "临床前肿瘤模型的免疫状况，以及研究治疗方法如何调节免疫状况。
OMIP-105: A 30-color full-spectrum flow cytometry panel to characterize the immune cell landscape in spleen and tumor within a syngeneic MC-38 murine colon carcinoma model
This panel was designed to characterize the immune cell landscape in the mouse tumor microenvironment as well as mouse lymphoid tissues (e.g., spleen). As an example, using the MC-38 mouse syngeneic tumor model, we demonstrated that we could measure the frequency and characterize the functional status of CD4 T cells, CD8 T cells, regulatory T cells, NK cells, B cells, macrophages, granulocytes, monocytes, and dendritic cells. This panel is especially useful for understanding the immune landscape in “cold” preclinical tumor models with very low immune cell infiltration and for investigating how therapeutic treatments may modulate the immune landscape.
期刊介绍：
Cytometry Part A, the journal of quantitative single-cell analysis, features original research reports and reviews of innovative scientific studies employing quantitative single-cell measurement, separation, manipulation, and modeling techniques, as well as original articles on mechanisms of molecular and cellular functions obtained by cytometry techniques.
The journal welcomes submissions from multiple research fields that fully embrace the study of the cytome:
Biomedical Instrumentation Engineering
Biophotonics
Bioinformatics
Cell Biology
Computational Biology
Data Science
Immunology
Parasitology
Microbiology
Neuroscience
Cancer
Stem Cells
Tissue Regeneration.
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