通过支撑液膜进行中空纤维-注射器平衡采样，以评估药物与血浆的结合。

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS Bioanalysis Pub Date : 2024-08-08 DOI:10.1080/17576180.2024.2377908

Thaer Barri, Ruba Ramzi, Nasir M Idkaidek, Nabil N Al-Hashimi, Faisal Al-Akayleh, Ahmed S A Ali Agha

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引用次数: 0

摘要

目的：采用中空纤维注射器支撑液膜平衡采样（HFiS ESTSLM）和 RP-HPLC 分析法评估药物与血浆的结合（DPB）。材料与方法：采用 HFiS ESTSLM 和 RP-HPLC 评估三种弱碱性药物（美托洛尔、苯海拉明和西地那非）的 DPB，这三种药物的亲水性和与血浆的结合能力各不相同。结果显示结果表明，三种模型药物的 DPB 值随药物的增加而增加。DPB 的这一趋势证实了 HFiS ESTSLM 具有测定药物 DPB 所需的灵敏度。在测试的药物浓度范围内，DPB 与药物浓度有关，尤其是在高浓度时。结论HFiS ESTSLM 和 RP-HPLC 为评估这些基本药物的 DPB 提供了一种简单、方便且经济有效的方法。

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Hollow Fiber-in-Syringe Equilibrium Sampling Through Supported-Liquid Membrane for Evaluation of Drug-Plasma Binding.

Aim: The aim was to evaluate drug-plasma binding (DPB).by employing Hollow Fiber-in-Syringe Equilibrium Sampling Through Supported Liquid Membrane (HFiS ESTSLM) and RP-HPLC analysis. Materials & methods: HFiS ESTSLM and RP-HPLC were used to evaluate DPB of three weak basic drugs (Metoprolol, Diphenhydramine, and Sildenafil) with differing hydrophilicity and binding ability to blood plasma. Results: The results exhibited an increasing drug-dependent magnitude of DPB for the three model drugs. This trend of DPB confirmed that HFiS ESTSLM has the required sensitivity for determining DPB of the drugs. The DPB was drug concentration-dependent within the tested drug concentration range, especially at high concentration. Conclusion: HFiS ESTSLM and RP-HPLC offered a simple, easy and cost-effective procedure to evaluate DPB of these basic drugs.

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.