Réka Svigruha, László Molnár, Károly Elekes, Zsolt Pirger, István Fodor
{"title":"三丁基锡暴露对软体动物模型物种 Lymnaea stagnalis 的胚胎发育和行为的影响。","authors":"Réka Svigruha, László Molnár, Károly Elekes, Zsolt Pirger, István Fodor","doi":"10.1016/j.cbpc.2024.109996","DOIUrl":null,"url":null,"abstract":"<div><p>The presence of the organotin compound tributyltin (TBT) in aquatic ecosystems has been a serious environmental problem for decades. Although a number of studies described the negative impact of TBT on mollusks at different levels, investigations connected to its potential effects during embryogenesis have been neglected. For a better understanding of the impact of TBT on mollusks, in the present study, embryos of previously TBT-treated or not treated specimens of the great pond snail (<em>Lymnaea stagnalis</em>) were exposed to 100 ng L<sup>−1</sup> TBT from egg-laying (single-cell stage) until hatching. According to our results, TBT significantly delayed hatching and caused shell malformation. TBT transiently decreased the locomotion (gliding) and also reduced the feeding activity, demonstrating for the first time that this compound can alter the behavioral patterns of molluscan embryos. The heart rate was also significantly reduced, providing further support that cardiac activity is an excellent indicator of metal pollution in molluscan species. At the histochemical level, tin was demonstrated for the first time in TBT-treated hatchlings with intensive reaction in the central nervous system, kidney, and hepatopancreas. Overall, the most notable effects were observed in treated embryos derived from TBT treated snails. Our findings indicate that TBT has detrimental effects on the development and physiological functions of <em>Lymnaea</em> embryos even at a sub-lethal concentration, potentially influencing their survival and fitness. Highlighting our observations, we have demonstrated previously unknown physiological changes (altered heart rate, locomotion, and feeding activity) caused by TBT, as well as visualized tin at the histochemical level in a molluscan species for the first time following TBT exposure. Further studies are in progress to reveal the cellular and molecular mechanisms underlying the physiological and behavioral changes described in the present study.</p></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"285 ","pages":"Article 109996"},"PeriodicalIF":3.9000,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1532045624001649/pdfft?md5=0141f6a19ec0cf187d02beaa9bc35580&pid=1-s2.0-S1532045624001649-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Effect of tributyltin exposure on the embryonic development and behavior of a molluscan model species, Lymnaea stagnalis\",\"authors\":\"Réka Svigruha, László Molnár, Károly Elekes, Zsolt Pirger, István Fodor\",\"doi\":\"10.1016/j.cbpc.2024.109996\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The presence of the organotin compound tributyltin (TBT) in aquatic ecosystems has been a serious environmental problem for decades. Although a number of studies described the negative impact of TBT on mollusks at different levels, investigations connected to its potential effects during embryogenesis have been neglected. For a better understanding of the impact of TBT on mollusks, in the present study, embryos of previously TBT-treated or not treated specimens of the great pond snail (<em>Lymnaea stagnalis</em>) were exposed to 100 ng L<sup>−1</sup> TBT from egg-laying (single-cell stage) until hatching. According to our results, TBT significantly delayed hatching and caused shell malformation. TBT transiently decreased the locomotion (gliding) and also reduced the feeding activity, demonstrating for the first time that this compound can alter the behavioral patterns of molluscan embryos. The heart rate was also significantly reduced, providing further support that cardiac activity is an excellent indicator of metal pollution in molluscan species. At the histochemical level, tin was demonstrated for the first time in TBT-treated hatchlings with intensive reaction in the central nervous system, kidney, and hepatopancreas. Overall, the most notable effects were observed in treated embryos derived from TBT treated snails. Our findings indicate that TBT has detrimental effects on the development and physiological functions of <em>Lymnaea</em> embryos even at a sub-lethal concentration, potentially influencing their survival and fitness. Highlighting our observations, we have demonstrated previously unknown physiological changes (altered heart rate, locomotion, and feeding activity) caused by TBT, as well as visualized tin at the histochemical level in a molluscan species for the first time following TBT exposure. Further studies are in progress to reveal the cellular and molecular mechanisms underlying the physiological and behavioral changes described in the present study.</p></div>\",\"PeriodicalId\":10602,\"journal\":{\"name\":\"Comparative Biochemistry and Physiology C-toxicology & Pharmacology\",\"volume\":\"285 \",\"pages\":\"Article 109996\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-08-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1532045624001649/pdfft?md5=0141f6a19ec0cf187d02beaa9bc35580&pid=1-s2.0-S1532045624001649-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Biochemistry and Physiology C-toxicology & Pharmacology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1532045624001649\",\"RegionNum\":3,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1532045624001649","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
几十年来,有机锡化合物三丁基锡(TBT)在水生生态系统中的存在一直是一个严重的环境问题。尽管许多研究都描述了三丁基锡化合物在不同水平上对软体动物的负面影响,但有关其在胚胎发育过程中的潜在影响的研究却被忽视了。为了更好地了解三丁基锡化合物对软体动物的影响,本研究将大塘螺(Lymnaea stagnalis)胚胎暴露于 100 ng L-1 的三丁基锡化合物中,从产卵(单细胞阶段)一直到孵化。结果表明,三丁基锡化合物会明显延迟孵化,并导致贝壳畸形。三丁基锡化合物可短暂降低胚胎的运动(滑行)能力,并减少摄食活动,首次证明了该化合物可改变软体动物胚胎的行为模式。心率也明显降低,进一步证明心脏活动是软体动物体内金属污染的极佳指标。在组织化学水平上,经三丁基锡化合物处理的幼体首次出现锡中毒现象,中枢神经系统、肾脏和肝胰脏都出现了强烈反应。总之,在经三丁基锡化合物处理的蜗牛胚胎中观察到了最显著的影响。我们的研究结果表明,即使在亚致死浓度下,三丁基锡化合物也会对莱姆蜗牛胚胎的发育和生理功能产生有害影响,从而可能影响其存活率和适应能力。为了突出我们的观察结果,我们展示了三丁基锡化合物引起的之前未知的生理变化(心率、运动和摄食活动的改变),并首次在软体动物中暴露于三丁基锡化合物后在组织化学水平上观察到锡的变化。目前正在开展进一步研究,以揭示本研究中描述的生理和行为变化的细胞和分子机制。
Effect of tributyltin exposure on the embryonic development and behavior of a molluscan model species, Lymnaea stagnalis
The presence of the organotin compound tributyltin (TBT) in aquatic ecosystems has been a serious environmental problem for decades. Although a number of studies described the negative impact of TBT on mollusks at different levels, investigations connected to its potential effects during embryogenesis have been neglected. For a better understanding of the impact of TBT on mollusks, in the present study, embryos of previously TBT-treated or not treated specimens of the great pond snail (Lymnaea stagnalis) were exposed to 100 ng L−1 TBT from egg-laying (single-cell stage) until hatching. According to our results, TBT significantly delayed hatching and caused shell malformation. TBT transiently decreased the locomotion (gliding) and also reduced the feeding activity, demonstrating for the first time that this compound can alter the behavioral patterns of molluscan embryos. The heart rate was also significantly reduced, providing further support that cardiac activity is an excellent indicator of metal pollution in molluscan species. At the histochemical level, tin was demonstrated for the first time in TBT-treated hatchlings with intensive reaction in the central nervous system, kidney, and hepatopancreas. Overall, the most notable effects were observed in treated embryos derived from TBT treated snails. Our findings indicate that TBT has detrimental effects on the development and physiological functions of Lymnaea embryos even at a sub-lethal concentration, potentially influencing their survival and fitness. Highlighting our observations, we have demonstrated previously unknown physiological changes (altered heart rate, locomotion, and feeding activity) caused by TBT, as well as visualized tin at the histochemical level in a molluscan species for the first time following TBT exposure. Further studies are in progress to reveal the cellular and molecular mechanisms underlying the physiological and behavioral changes described in the present study.
期刊介绍:
Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.